From a contaminated intravenous catheter you isolate Gram-positive cocci, beta-hemolytic, and catalase positive. MSA results below (grown aerobically): Which pathogen from the list below have you isolated? O Streptococcus pyogenes_ O Staphylococcus aureus_ Staphylococcus epidermidis Streptococcus pneumoniae_
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- Gas Gangrene: You are working in the Surgery Unit and are helping a paitent with "gas gangrene". Samples of tissues and fluid delivered to lab for culture and identification. Microbes could not grow aerobically; growth occurred anaerobically only Blood agar after anaerobic incubation (below), note zones of clearing around colonnies:What is this organism? And what other test could be done to confirm it's identification? 1.Gram stain - positive cocci, chains Catalase - weak positive Hemolysis - beta BE - positive NaCl- positive Bacitracin - no zone of inhibition PYR - positive Answer: Enterococcus spp. CAMP test to verify the identity 2.Gram positive cocci, chains Catalase - negative Hemolysis - alpha BE - negative NaCl - negative P disk - resistant Bile Solubility - negative Answer - viridans group, Use PYR test to verifyYou are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flask
- A newborn presents with sepsis. Blood samples are taken and sent for culture. Gram stain, blood agar plates and catalase results below. VP test + Gram stain round cells ~1 micrometer diameter Blood agar:Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient AgarSITUATION: A 70-year-old retired welder who is a heavy smoker arrives to the emergency room suffering from fever and shortness of breath for the last three days. He is also suffering from cough with rust-colored sputum. The chest X-ray result reveals involvement in the left lower lobe of his lungs. The Gram staining results from his sputum and blood which were cultured indicates the presence of gram-positive streptococci. He was checked into a hospital and placed on penicillin. Two days later the patient’s health improved. a. What organism is responsible for the patient’s infection?b. Why is the patient at high risk for becoming infected with bacterial organisms?c. How could the patient likely have prevented contracting the disease?d. If the sputum and blood samples are plated on blood agar, what type of hemolytic reaction should be seen?
- A Gram stain result from cerebrospinal fluid (CSF) indicates Gram positive cocci (G+C) in pairs. Which of the following would be the presumptive identification: O Haemophilus influenzae O None of the answers apply O Streptococcus pneumoniae O Streptococcus pyogenes O Neisseria meningitidis O E. coli O Staphylococcus aureusBased on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.What is the microbiology laboratory test that Identifies Pseudomonas maumuensis and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.
- Which of the following bacteria is the most common isolate from blood? Vibrio cholerae Mycobactererium tuberculosis Mycoplasmapneumoniae Helicobacter pylori Escherichia coli Which of the following bacteria often cause Otitis media (middle ear infections) in children? Mycoplasma pneumoniae Mycobactererium tuberculosis Streptococcuspneumoniae Helicobacter pylori Escherichia coli Which of the following influenza viruses is/are influenza B virus(es)? H1N1 H3N2 H7N9 All of the above None of the aboveWhich potential pathogen has been isolated? Staphylococcus aurues Streptococcus pyogenes/GAS Strotociccus mutans Streptococcus pneumoniaeStreptococcus pyogenes, the main causative agent of bacterial pharyngitis (aka “Strep throat”) is differentiated from other Streptococcus species by type of hemolysis; S. pyogenes is ______ hemolytic on a Blood Agar Plate (BAP).