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Genetics Q2
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Solved in 3 steps
- Explain how Meselson and Stahl showed that DNA followed the semi- theory conservative.Describe the Meselon -Stahl experiment Include how they distinguished new from old DNA strands, what the centrifuge was for, and how it showed semi-conservative replication.What is the purpose of precipitation? Why should the ethanol/lysate boundary not be disturbed? What information do learn from the results of the DNA extraction?
- Meselson and Stahl grew bacteria in medium containing heavy nitrogen. After many celldivisions, the bacteria were removed, washed and allowed to continue growth in mediumcontaining normal (light) nitrogen.a. In this experiment, what part of the DNA gets labeled with heavy nitrogen?What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol? For reference, this is the procedure for the experimentWhen Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteria
- With regard to the experiment described in Figure, The DNA extract was treated with DNase, RNase, or protease.Why was this done? (In other words, what were the researcherstrying to determine?)Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.In your extraction of DNA from wheat germ, would you expect that nuclear DNA is the only source of DNA in your sample?
- Why should the ethanol/lysate boundary not be disturbed? What information do learn from the results of the DNA extraction?What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?Which of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling b.metacoiling c. ultracoiling d. telephone coiling
