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- The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109erythrocytes will form a monolayer of area 0.89 m2when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2in area. Show that these data can be accounted for only if the erythrocyte membrane is a bilayer.Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs? Please explain in detail the main findings with your own words.Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs? Make a list of it.
- Example Problem: FRAP Data Interpretation The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity ROI A 3 Time B Time Post-bleaching imaging с Time D Time Pre-bleaching Bleaching imaging imaging (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109 erythrocytes will form a monolayerof area 0.89 m2 when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2 in area.Show that these data can be accounted for only if the erythrocyte membraneis a bilayer.Example Problem: FRAP Data Interpretation The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity ROI A 3 Time Pre-bleaching Bleaching imaging imaging B Time Post-bleaching imaging C Time D Time (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
- Insert Draw X 6 7 of 8 FI Arial BI Layout Design V I U ab x₂ 585 words 11 x, x² F2 References A A Aa Po V ADA Type of Transport English (United States) 20 F3 ECF Na IK pump O Cytosol === E 5. When given images such as the ones below, be able to identify the specific type of cell transport shown. Practice using this online interactive activity. Carrer protes Mailings Review 1:3 15- V 000 000 F4 Image Guc View Tell me F5 2 I ¶ ✔ ABbCcDdEe Normal MacBook Air F6 AaBbCcDdF AaBbCcDc AaBbCcDdEe AaBb( No Spacing Heading 1 Heading 2 Title Type of Transport F7 Focus ► 11 3 F9 AMAZIN E REE F8 VEEEE Share Styles Dictate Pane F10 E Editor + 212Cell growth kinetics A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is monitored with the following results. Time (days) Cell concentration (cells.ml* x 10°) 0.0 0.45 00.2 0.52 0.5 0.65 1.0 0.81 1.5 1.22 2.0 1.77 2.5 2.13 3.0 3.55 3.5 4.02 4.0 3.77 4.5 2.20 (a) Determine the specific growth rate during the growth phase. (b) What is the culture doubling time?Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs? Please explain in detail
- 5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a small region of the cell membrane with an intense laser light, and determine the time it takes for the bleached spot to recover fluorescent signal (see figure below). (a) (b) Bleach Laser bleaching of fluorescent marker Fluorescence intensity recovery Recovery Time How would the recovery time of your protein change if the membrane contained a higher concentration of unsaturated fatty acids? Why? How would the recovery time of your protein change if you conducted the experiment at a lower temperature? Why? How would the recovery time of your protein change if it were anchored to the membrane skeleton? Why?For most cell types, serum must be added to the media to get cells to grow and proliferate in culture. Nevertheless, serum is very expensive and can complicate experiments utilizing the cells. Many companies have developed "artificial serum" which enables cells to multiply without the use of serum. See data below. What are likely components of the artificial serum that enables cells to grow. Explain (limit 3-4sentences). Non 2% Human plasma 1% Artificial serum(XF) 1% Artificial serum(AF) 104 cells/cm² Cell density cultured after 4 days 127 10- 2 0 NON 2% 1% 1% Human Artificial Artificial plasma serum(XF) serum(AF) PDL [times] 30- 25+ 20- 15- 10- 5- 0 -2% Human plasma 0 Subculture 1% Artificial serum (XF) 1% Artificial serum(AF) 5 10 15 20 25 30 Culture days Medium: HFDM-1 (+) was used.Please answer the question. No need to provide a side information, even The calculation it self would be fine. :)