Q: What is the effect of light on microbial growth?
A: Introduction:- The quantity of bacteria in a population, rather than the size of individual cells,…
Q: . If you wanted to determine the number of endospores in a sample of soil, what could you do before…
A: Endospores are defined as the differentiated cell formed or produced within cells of a particular…
Q: Why is algae being grown in wastewater
A: INTRODUCTION Production and emission of CO2 from different sources have caused significant changes…
Q: What is agar and why is it necessary for growing bacteria on plates?
A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…
Q: Some exconjugants did not grow on minimal medium. On what medium would they grow?
A: Exconjugants can be described as bacterial cells, which separates following the conjugation. A…
Q: Based on your observations of microorganisms in their living state, which between the wet mount…
A: the hanging drop method is better because it allow for longer time visualisation .
Q: ILLUSTRATIONS: 1. Sterilizers seen in the laboratory and their parts.
A: 4 main methods of sterilizarion - physical methods Radiation method Ultrasonic method Chemical…
Q: How does bacteria grow on agar plates?
A: Bacteria are the most primitive organisms on Earth. They can survive in most extreme environments as…
Q: If any of the water sample plates had a lawn or too many colonies to count, what might you have…
A: A technique known as the standard plate count is used for estimation of the population density in…
Q: How does the UV radiation control the microbial growth affect of cells?
A: It is required to describe the affect of ultraviolet radiation controlling the microbial growth of…
Q: Why is it important to become familiar with the microbiology laboratory's apparatus and equipment?
A: You should always know your lab equipment well before you do any type of experiment because without…
Q: What effect do you think using a greasy, oily, dirty slide will have on your judgement of the Gram…
A: Bacteria are the most important microorganisms to the food processor. Some bacteria are beneficial…
Q: How could you determine whether the turbidity in your LB tube was from a mixture of different…
A: Introduction: The term "turbidity" describes how cloudy a solution is. Turbidity in a Luria Broth…
Q: What factors influence the appearance of microbial colony
A: Microbial colony can be defined as the visible mass of microbes like fungi ,bacteria etc on a…
Q: Why after a certain period the growth rate of the culture decreases until growth stops?
A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…
Q: What happens to microbes that encounter temperatures belowtheir minimum and above their maximum…
A: Micro-organism are tiny organisms that cannot be seen by the naked eye, but are visible under…
Q: When observing the endodpore slide in the microscope, what is the difference between endospore and…
A: In spore staining the dye, Malachite green is used for staining the spore, and steaming is used as…
Q: What are the advantages and disadvantages of observ-ing living-specimen slides and…
A: Microorganisms are the organisms that are not visible to the naked eye. Their size ranges in few…
Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…
A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…
Q: How would a change in the temperature at which something was incubated affect the amount of mold…
A: The differential growth patterns of organisms are due to the temperature sensitivity of the enzymes.…
Q: among these five areas such as: air, faucet, doorknow, table, and floor, in the laboratory is…
A: Microorganisms are present everywhere in nature, they are ubiquitous in their dwelling nature ie…
Q: Why did the soap have to “cure” in the molds?
A: Fats and oils are triglycerides having a glycerol backbone with all the hydroxyl groups esterified…
Q: How is the presence or absence of viable endospores related to sterility?
A: Endospore is a resistant asexual spore that develops inside the bacteria under the extreme…
Q: What are the difference in the movement of microorganisms under wet mount from hanging drop…
A: There are some plus point for both the techniques like the wet Mount and hanging drop method…
Q: If you do not see single colonies on some of the plates, explain what might have happened to the…
A: A bacterial colony is a mass of bacterial cells originating from mother cells, they grow on solid…
Q: What information can obtain from observing the growth of bacteria on solid agar?
A: Agar is a jelly-like substance, obtained from rhodophyceae that are commonly knows as the red algae.…
Q: Which environment has many microorganisms and which has few?
A: Microorganisms live in every kind of habitat and their presence invariably affects the environment…
Q: What does a MEGA plate tell you about your microbial culture?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: who introduced the use of cotton plug in microbial culture tubes.
A: Cotton plugs are utilized in bacterial specimens and culture mediums. The cotton plugs' primary role…
Q: Why must you first clean debris from items before beginning disinfection or sterilization? (
A: Until the discovery of the microbes by the famous scientist Louis Pasteur, one of the signs of the…
Q: A novice microbiology student incorrectly explains that immersion oil increases the magnification of…
A: The function of immersion oil is to increase the resolving the power of the light microscope. The…
Q: What compounds or chemicals might be added to a medium to make it selective?
A: Culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth of…
Q: Which bacterial species exhibited true motility on the slides?
A: Bacteria are single-celled prokaryotes ubiquitous in nature. As such, they can be found in different…
Q: What types of bacteria grow on nutrient agar?
A: Nutrient agar is a nutrient-rich medium that allows the growth of a wide range of organisms in the…
Q: What appears to have been the limiting factor for microbial growth and activity in soil?
A: Soil micro-organisms are a very crucial part of the soil. They have several roles to play:…
Q: What type of agar would you use to grow gram negative bacteria
A: Gram-negative bacteria are the bacteria that do not consist of the crystal violet stain and they are…
Q: methods will you perform to collect and identify the microorganisms suspended in the indoor air?
A: Air sampling is the technique by which quality control lab associated with pharmaceutical,…
Q: Based off Pasteur’s sterility experiment, which of the following choices is least likely to result…
A: Louis Pasteur was the scientist who invented pasteurization, which is a technique to kill…
Q: In a tabulated form, differentiate the physical and chemical methods of sterilization.
A: The microbiological technique that is performed for the removal, killing, or deactivation of…
Q: agnification is required to observe microorganisms?
A: A microorganism, often known as a microbe, is a microscopic organism that can be a single cell or a…
Q: When the microbes were exposed to different temperatures how was it possible to see minimal growth?…
A: Microorganisms are divided into different groups depending on their optimum growth temperatures.…
Q: Turbidometry is a method used for counting microbes?
A: Microbiology is the branch of biology that deals with microorganisms. Bacterial cultures and their…
Q: Write a note on the measurement of growth of microorganisms, factors influence microbial growth and…
A: Microbial growth can be defined as the growth of a population or an increase in the number of cells,…
Q: How would light shone on one side of the Winogradsky column influence the growth of microorganisms?
A: Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and…
Q: discuss the limitations in using the light microscope to study microbial communities.
A: Microscope is the most basic and useful instrument used in the microbiology laboratory. It is…
Q: what microbial features would you use a scanning electron microscope and transmission electron…
A: Scanning electron microscope special features- projects 3D image of surface Appearance of the…
Q: What are the factors that influence the efficiency of chemical sterilization?
A: Chemical Sterilization is the process of removal of microorganisms by the use of chemical…
How would light shone on one side of the column influence the growth of microorganisms?
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- When the microbes were exposed to different temperatures how was it possible to see minimal growth? What had to be functional in order for growth to occur?What magnification is required to observe microorganisms?what microbial features would you use a scanning electron microscope and transmission electron microscope to observe.
- What are the disadvantages of doing a total microbial count by examining wet mounts under the microscope?You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. Based on this information answer questions 1-10: 6) What would be the last reagent you use to identify the bacteria?7) What type of visual aid you need to help you see the bacterial samples clearly?8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria?Did each handwashing treatment result in similar types of organisms growing on the plates? (look at the colonies and gram types) Explain why or why not?
- An immersion lens was used to examine the microorganisms microscopically in a light microscope. ANSWER THE QUESTIONS 1. What symbols can be used to identify an immersion lens? What is the immersion system? 3. Why is it necessary to use an immersion lens to study microorganisms? 2.Describe the production process of wine, highlighting the role of the microorganisms involved and stating the economic, health, and nutritional benefits. (2 pages)d) Since we know this bacterial organism thrives in colder areas of the world, what can be said about the effect of temperature on the growth patterns ofthis bacterial organism? How would increasing the temperature affect the growth constant? e) If the bacterial organism is resistant to antibiotics, how would administering antibiotics affect the growth of this organism?
- discuss the limitations in using the light microscope to study microbial communities.why is it important to understand the morphology of microorganismsWhich among these five areas such as: air, faucet, doorknow, table, and floor, in the laboratory is expected to have the most number of microorganisms? Provide an explanation. Based on surface and consistency of the observed colonies from the representative plates, which among the areas may contain potentially pathogenic microbes? Provide an explanation.