Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
How would you determine if a transfection was transient or stable?
This image might be helpful
![Overview of Transfection
Transfection is the means of inserting genetic materials (DNAS/RNAs) into mammalian cells.
The main purpose of transfection is to study the function of genes or gene products, by
enhancing or inhibiting specific gene expression in cells, and to produce recombinant proteins
in mammalian cells [1].
There are two types of transfection:
1. transient transfection: the foreign gene exists in the cytoplasm where they are expressed for
a period of time
2. stable transfection: the foreign gene is somehow incorporated into host genome allowing a
sustainable expression. In this case, a new cell line has been created.
Which of these occurs, is often a matter of chance.
There are three main methods of transfection [2]:
a. Biological: eg. virus mediated transfection also known as transduction
b. Chemical: most widely used method. The genetic material forms a complex with another
chemical, which then is carried into the cell by endocytosis/ phagocytosis. Eg. Liposome
mediated, dendrimer mediate, etc.
c. Physical: eg. Electroporation, biolistic particle delivery with gene gun, direct microinjection
and laser based transfection.
There are advantages and disadvantages associated with each of these methods. The choice of
the method depends on the scope of the research.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc19bf035-a1ae-4f82-b14b-8e7cb737e08e%2Feca63c01-f581-4818-b3b2-3bfca31c7de2%2Fr73j7em_processed.jpeg&w=3840&q=75)
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
