HPLC Detector signal Q3: The Figure shown a HPLC chromatogram of two compound .Calculate the following values using the data in this chromatogram. 06- a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 05 03 لحرية 02 0.1 تختبر تحليل الى 45.0 s void 180.0 s uracil 247.5s guanine

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