I II II Methanol (mM) Rate of Reaction (mMmin-1) Rate of Reaction in presence of 5.0 µM Ethanol 0.50 23.5 16.67 1.0 32.2 25.25 1.5 36.9 30.49 2.5 - 41.8 37.04 3.5 44.0 38.91 (Nelson, 2008)
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11) Using the data above, construct Lineweaver-Burk plots. First, graph the inverse of the reaction velocity (
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- Determine the Ki for the inhibitor at 30 °Cand decide what type of inhibitor is being used. Eo T I S V (g/I) (°C) (mmol/ml) (mmol/ml) (mmol/ml-min) 1.6 30 0.1 2.63 1.6 30 0.033 1.92 1.6 30 0.02 1.47 1.6 30 0.01 0.96 1.6 30 0.005 0.56 1.6 49.6 0.1 5.13 1.6 49.6 0.033 3.70 1.6 49.6 0.01 1.89 1.6 49.6 0.0067 1.43 1.6 49.6 0.005 1.11 0.92 30 0.1 1.64 0.92 30 0.02 0.90 0.92 30 0.01 0.58 0.92 30 0.6 0.1 1.33 0.92 30 0.6 0.033 0.80 0.92 30 0.6 0.02 0.57Aerobic degradation of an organic compound by mixed cultureof organism in wastewater can be represented by following reaction. C3H6O3 + a O2 + b NH3 → c C5H7NO2 + d H2o + e CO2 A. Determine a, b, c, d and e, if YX/S = 0.4 d X/g S. B. Determine the yield coefficients YX/O2 and YX/NH3. C. Determine the degree of reductions for the substrate, bacteria and RQ for the organismsThe following values were taken from the Richard's table on the thermal death kinetics of B. stearothermophi. cal/mol K): (A=4.9x1037min', E=67.48 kcal/mol, R=1.987 Temperature, °C rate The cumulative del factor Specific constant k, min death -1 V 120 1.47 7.25 121 1.83 9.08 125 4.35 22.08 130 12.55 65.10 (i) The time taken for heating, cooling and holding periods in the sterilisation of a batch fermentor medium for the destruction of B. stearothermophilus were: time to heat from 100 to 121°C = 30 min time to cool from 121 to 100 °C =16 min time of holding at 121 °C = 12 min Calculate the destruction ratio (No/N) for the process. (ii) If the sterilisation were to be carried out continuously at 130 °C, and the heating and cooling characteristics were: time to heat from 100 to 130 °C = 2 min time to cool from 130 to 100 °C = 1 min what holding time would be required to achieve the same ratio of (No/N) as in section (i) above? State all the assumptions made in your calculations.
- 1. Determine the Michaelis-Menten parameters of Vmax and Kỵ for the reaction S+E E.S E.S+W P+E The rate of reaction is given as a function of substrate: [S] 0.2 0.02 0.01 kmol/m³ -I'S kmol/m³.s E.S k₂E+S 0.54 0.28 0.19 0.005 0.1 0.002 0.045Discuss the suitability of bromocresol green and thymol blue (2nd change only) for use as indicators in an acetic acid - sodium hydroxide titration. Paragraph в I A + v ...Calculate the reduction potential of cytochrome a3 (°' = 0.385 V) at 25.0°C when the Fe3+ form = 87.0 μM and the Fe2+ form = 25.0 μM. 0.440 V 0.353 V 0.417 V 0.387 V
- 2 NDC 0186-0743-31 Keep containigdy closed. PRILOSec Protect from light and moisture Store between 15°C and 30°C (599 and 80°F) OMEPRAZOLE) 30 Delayed-Release Capsules 40 mg Rx only Manufactured for AstraZeneca LP PROSE Wington, DE 10850 40 By MERCK & CO. Whitehouse Shanon, NJ 06809, USA Name AstraZeneca 11 0.4 ESCOFF Figure 6-A2 Drug label for Prilosec 1) Read the drug label in Figure 6-A2. How many Prilosec (omeprazole) capsules would contain 0.32 g of the drug? 2) Using a formula estimate the body surface area of a person whose height is 6 feet 3 inches. and who weighs 200 pounds. |||| 0.5 USUAL ADULT DOSAGE: See package insert NDC 0054-3542-58 The PROSEC (omeprazole) Delayed Release Capsule should be swallowed whole and not opened, chewed, or crushed. 4) Order: Oncovin (vincristine sulfate) 2.5 mg po daily. The BSA is 1.63 m2 and the recommended dose is 1.4 - 2.0 mg/ m2. Is the prescribed dose in the safe dose range? 5) Indicate with an arrow a reading of 0.42 mL on the scale…Which of these heterocyclic drugs is likely to be the least soluble in water? Use the Fsp³ parameter to decide. OH Tramadol Chemical Formula: C16H25NO2 YOUR OW Pantoprazole Torasemide Chemical Formula: C16H15F2N3O4S Chemical Formula: C16H20N4O3S Temazepam -OH Chemical Formula: C16H13CIN₂O2 Tioconazole Chemical Formula: C16H13C3N₂OS A. Tramadol B. Pantoprazole C. Torasemide D. Temazepam E. ToconazoleThe energy of activation for the reaction 2 HI – H2 + I2 is 180. kJ•mol-1 at 544 K. Calculate the rate constant using the equation k = Ae-EalRT. The collision diameter for HI is 3.5×10-8 cm. Assume that the pressure is 1.00 atm. 4.0 4.510e-27 X M-1.s-1
- Figure I shows the Michaelis Menten plot of initial reaction velocity (as percentage of Vmax) versus [S] (concentration) for the carbonic anhydrase reaction in the absence and presence of the inhibitor acetazolamide. Carbonic anhydrase participates in regulation of the pH and bicarbonate content of a number of body fluids. 100 No inhibitor Acetazolamide 0.2 0.4 0.6 0.8 1 [S] (mM) Figure 1 (i) Compare Vmax and Km of the enzyme without inhibitor and in the presence of acetazolamide. Determine the type of inhibition shown by acetazolamide. Explain your answer. (ii) Name TWO (2) other types of inhibitions besides the inhibition shown by acetazolamide in Qla)(i). List down the kinetic properties of these inhibitions. Sketch a graph of I/V versus 1/[S] showing plots in the absence of an inhibitor and in the presence of the types of inhibitors mentioned in Qla)(ii). (iii) V (% of VmaxRegarding the reasoning for the Michaelis-Menten equation to be unsuitable for accurate analysis of experimental data, select all that apply: It is nonlinear It is only valid for reactions at equilibrium It is not valid under experimental conditions Extrapolation to Vm is inaccurate and therefore Km also cannot be accurately describedIn cation exchange chromatography, positively charged counter-ions will be bound to the stationary phase. O True O False