If 37% of a cell's nucleotides are cytosine, which of the following would also be true? *You may use the calculator function on the sidebar to help with the math. O 13% are adenine O 26% are thymine O 74% are guanine O 74% are adenine
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- Composition as a mole fraction of one of a double-stranded DNA strand T = 0.22 and C = 0.30. In the light of this information, the following values are Calculate as a fraction. If the given information is used to calculate the desired value, If it is not sufficient, indicate the result as X.Make your DNA extraction buffer. Add 2 teaspoons of detergent, 1 teaspoon of salt, and ½ cup of water to one of the plastic cups. Stir well. 4 Open the bag of fruit and add 2 teaspoons of your DNA extraction buffer. What are cell membranes made of? Which parts are hydrophobic and which are hydrophilic? What does soap do to cell membranes? Why would this be helpful in extracting DNA? What function does the salt perform in the solution?STRUCTURE AND NOMENCLATURE BASE FROM THE PICTURE ANSWER THE FOLLOWING: ( I ALL READY ANWER IT BUT I'M NOT SURE WITH MY ANSWERS, PLEASE CORRECT MY ANSWER IF ITS WRONG. THANK YOU.) Give the ABBREVIATED NAME of the nucleotide in the 5' end. MY ANSWER IS AMP Name the fourth nucleotide from the 3' to 5' end. Use the correct/appropriate punctuations, spacing and letter case based on the nomenclature. MY ANSWER IS Cytosine-5'-monophosphate What is the total number of hydrogen bonds formed from the complex of the given structure? Give ONLY the NUMBER. MY ANSWER IS 10 How many 3',5'-Phosphodiester linkages were formed? from the complex of the given structure? Give ONLY the NUMBER of the linkages. MY ANSWER IS 3 Give the ABBREVIATED NAME of the nucleotide in the 3' end. MY ANSWER IS U Name the first complementary base from the 5' direction. USE ALL CAPITAL LETTERS ONLY. MY ANSWER IS ADENOSINE-5'-MONOPHOSPHATE How many terminal phosphate/s is/are present in the given structure? Give ONLY the…
- Given the following eukaryotic DNA strand, transcribe and translate the DNA into a polypeptide using the 3’ – 5’ strand as the template. You may use drawings, diagrams, colours and annotations to describe how the DNA strand will be synthesized into a functional protein 5’ - TATAAAAASSMSBMDATGSBDCCMBDBAATBSMDSTGTGTCCTMSBAG – 3’ (KEY: The letters SBMD are “made up” nucleic acids that depict non-coding regions in the DNA, hypothetically S pairs with B and M pairs with D).Each group will be provided with 20 g double-stranded DNA oligomer in STE buffer (0.1M NaCl/ Tris/ 10 mM EDTA, pH 7.4). The sequence of one strand of the oligomer is: 5’-AATGGTACGTATACTTTAT-3’ - show a table of % denaturation of DNA at different temperatures, using the equation provided in “Calculation” - plot a graph of % denaturation of DNA vs different temperatures with clear label and title, and hence determine the experimental Tm of the DNA sample - calculate the theoretical Tm of the sample using Marmur and Doty equation, compare the experimental Tm with theoretical Tm of the DNA sampleSTRUCTURE OF DNA Ueing the complementary base pairing rules in DNA, complete the following base pairs: Adenine - Cytosine -i Guanine -i Thymine - : Adenine : Cytosine :: Guanine :: Thymine 1
- Abosrbance (A 280) You are interested in isolating an enzyme Ribonuclease. Inside cells ribonuclease self-associates and can exist as dimers or trimers. The active form of the enzyme is monomeric with molecular weight of ~ 14Kda. In order to isolate ribonuclease, you use size exclusion chromatography column and obtain three peaks. Indicate which peak (starting from left to right) corresponds to Monomer (M), Dimer (D) and Trimer (T) forms of ribonuclease. PEAK I: PEAK II: PEAK III: Time M м Fractionsprateins OP (weak bonds) a better source of energy than H,O (strong bonds)? 37. What is the difference between AG, AG, and AG? Which is preferred by biochemists? 38. How can a reaction with a positive AG (like malate-> oxaloacetate) go forward in the cell? 46. Which tautomeric form of each base (A, T, G, and C) is found in standard DNA? What can be the result if the uncommon forms are incorporated into DNA? REstem-loop For each of the following, identify whether it is an example of primary, secondary, or tertiary structure that might be observed in a nucleic acid (such as DNA). Answers may be used more than once. nucleotide sequence B form supercoiling kinetochore of a yeast chromosome nucleosome secondary structure primary structure secondary structure tertiary structure tertiary structure secondary structure
- 5 pts Question 9 95 58 40 HS HS SH 65 8 M urea and 72 B-mercaptoethanol 65 Hs84 Hs- SH 72 26 HS 110 SH 84 26 110 95 124 40 58 Native ribonuclease Denatured reduced ribonuclease Figure 2.53 Biochemistry, Seventh Edition O 2012 W. H. Freeman and Company The image above shows how Anfinsen unfolded ribonuclease for his classic experiment to see if proteins contain all necessary information for adopting their folded states on their own. To achieve proper refolding and ribonuclease activity, Anfinsen must have: trick question: ribonuclease cannot adopt its folded state without the help of cellular machinery removed the mercaptoethanol followed by the urea very carefully removed both the urea and mercaptoethanol at the same time removed the urea followed by the mercaptoethanolThe Basic Structure of DNA 1. Nucleotides are the monomer subunits of DNA polymers. Review the structure of a nucleotide by labeling the three main parts: the sugar, the phosphate group and the nitrogenous base of the one shown below. NH2 N- || ОН 2. Which part (sugar, phosphate, or nitrogenous base) of the four types of nucleotides differ? 3. Based on the complementary base pairing rules we know that: A(denosine) pairs with and that G(uanine) pairs with 4. What two types of bonds are found in a DNA molecule and where are they found? а. bonds are found b. bonds are found O=a-oDrawing of DNA Structure Next, draw the structure of your double-stranded DNA molecule with circles representing the beads you used. Label each circle with the color bead: W for white, R for red, Y for yellow, O for orange, B for blue, and G for green. Also include the hydrogen bond barbells. a. Draw your double-stranded DNA structure here:
