Q: When a company changes from the LAL assay to the Recombinant Factor C assay, they must perform a…
A: LAL or Limulus amebocyte lysate assay is a technique used to assess and quantify the presence of…
Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…
A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…
Q: How does an RT-PCR work?
A: RT- PCR is a technique used in genetic studies used for the detection and quantification of mRNA.…
Q: Do you think a U-tube could be used to distinguish betweentransduction and transformation?
A: Transformation is the process of horizontal gene transfer, where the genetic material moves from one…
Q: Although many cloning applications involve introducing recombinant DNA into bacterial host cells,…
A: Cloning can be defined as the process by which identical copies of the organisms are created. More…
Q: Why is it difficult in a single experiment to transfer a largenumber of genes to a recipient cell…
A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…
Q: What phase of PCR (exponential, linear, or stationary) is analyzedto quantitate the amount of DNA or…
A: The genetic entity of the organism is mainly composed of nucleic acids. The nucleic acids DNA and…
Q: Describe a method of labeling a DNA probe. Explain how this probe maybe detected after DNA…
A: The probe is basically a single stranded sequence of DNA or RNA which is used commonly for the…
Q: In rRT–PCR, why do need to convert RNA to DNA first? Why can’t you amplify the RNA molecule…
A: Taq polymerase does not work on RNA samples, so PCR cannot be used to directly amplify RNA…
Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…
A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…
Q: Explain The replica plating technique?
A: Replica plating is used in the microbiological experiment. It helps in the testing of various…
Q: Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro?
A:
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Microbiology is the branch of science that deals with the study of microorganisms that are too small…
Q: Why is PCR beneficial?
A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…
Q: which is the delivery techniques can bbe used to best transfer recombinant plasmids into E.coli…
A: ANSWER) The process of transferring the recombinant plasmids into the E.coli cells is called…
Q: Can we do PCR on a plasmid?
A: In molecular biology, the plasmid is most abundantly used. The plasmid is a circular autonomously…
Q: In gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far…
A: Transduction is a process used to map the bacterial genes. The transduction is of two types,…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: In the conventional amplification technique of PCR (polymerase chain reaction), the amplified…
Q: Coomassie dye is the equivalent of what component used in analysis of DNA by agarose gel…
A: Western blot helps to identify specific proteins from a mixture of proteins and this step follows…
Q: By seeing the picture, how are Recombinant DNA formed? What is the difference between genetic…
A: Introduction: Genetic engineering or genetic modification or genetic manipulation involves direct…
Q: To maximize the number of mutations following UV irradiation, should you incubate the irradiated…
A: A mutation is a modification that happens in the deoxyribonucleic acid (DNA) sequence, either due to…
Q: What are the reagents used for Reverse Transcriptase PCR (RT-PCR) and Reverse Transcriptase–Real…
A: Introduction Reagents are substances used for the detection or determination of another substance,…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: The amplification of the DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) can be performed by…
Q: What is artificial transformation used for?
A: Transformation is a process in which an organism acquires exogenous DNA. This process occurs in two…
Q: Explain what is Flow cell DNA sequencing ?
A: What is DNA sequencing? It is the process of finding out the order of nucleotides i.e. adenine,…
Q: Describe the steps, starting from an endonuclease digested DNA sample, to complete a Southern blot…
A: Southern blotting is a hybridization method for identifying a specific DNA sequence in the host DNA.…
Q: Would it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?
A: Any group of Viruses that attach to the bacteria and infect the bacteria is known as bacteriophage.…
Q: . In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would…
A: Introduction: PCR(Polymerase Chain rlReaction) is a technique used to amplify a single copy or a few…
Q: How is DNA amplified in a polymerase chain reaction(PCR) procedure?
A: Step 1 Polymerase chain reaction (PCR) sometimes also called molecular photocopying is a technique…
Q: In DNA cloning, why do we need to check colonies for insert after transformation?
A: Gene is the basic unit of heredity. All living organisms contain certain nucleic acids as their…
Q: How does RT-PCR differ from traditional PCR?
A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…
Q: distinguish between bacterial cells that obtained the plasmid and those that did not
A: Bacteria are single celled organisms with a unique internal structure.
Q: Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells…
A: An example of a reporter gene is the Lac Z gene, this gene encodes for fluorescent proteins in the…
Q: Describe the technique of in situ hybridization. Explain how it can be used to map genes.
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Gel Electrophoresis Separates DNAFragments According to which thing?
A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…
Q: blue-white colony selection method in the recombinant colony screening
A: Blue - white screening for selection recombinat colony :- Blue - white screening is a rapid and…
Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to
A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…
Q: how can PCR products be labeled using fluorescence?
A: Polymerase Chain Reaction (PCR) is a molecular biology technique that is used for various purposes…
Q: Can you explain how PCR( Polymerase Chain Reaction can detect very) low amounts of DNA?
A: Polymerase Chain Reaction (PCR) is a molecular biology technique that is used to amplify a copy of…
Q: Vhat is needed from the cells for PCR?
A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…
Q: What determines the size (length) of the primary PCR product? What might a successful gel check of…
A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene of…
Q: What are five variables to consider when designing a primer for standard PCR and state why.
A: Polymerase chain reaction involves amplification of desired DNA strand using three steps i.e..…
Please help me answer topic is about making a recombinant DNA model
Step by step
Solved in 2 steps
- 9. During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification 11. What is not true for Sequence tagged site (STS) markers: cannot be mapped by fluorescence in situ hybridization (FISH) subset of STS markers are known as expressed sequence tag (EST) markers can readily be screened by a PCR assay short DNA sequences that occur at a unique location in the genome1. How can site-specific recombination be used in recombinant DNA technology? Answer and explain comprehensively.Pls Help me. 2. If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type of medium will be used? How will it work?
- 11. Discuss if you could perform the PCR and then agarose gel electrophoresis using a coding gene instead of a VNTR.What information and materials are needed to amplify region of DNA using PCR?1. Compare Illumina/Pacbio/Nanopore sequencing methods.2. How do you construct the profile matrix? Answer all the part in details and with proper examples.
- 1. What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger printing. 3. What is/are the sample that can be used for DNA finger printing? 4. Who is the culprit base on our interactive laboratory experiment?2. Please design a kit to replicate plasmid DNAS in the test tube. DNAFirst picture: 1. What must be added in a minimal media for the recombinants to grow? 2. What must be excluded in a minimal media for the non recombinants to be killed? Second picture: 1. How is the recipient cell different at time D than it was at time A? Choice 1:It has a greater number of genes Choice 2: It has a greater mass of DNA. Choice 3:It contains different genes Choice 4: It contains bacteriophage DNA. 2. At which time can the recipient cell first be described as "recombinant"? A,B,C, or D 3. Which of the following processes is responsible for the shape of the curve at time B? Choice 1:transduction Choice 2:another choice Choice 3:entry of Hfr DNA into the recipient cell Choice 4:entry of DNA from the recipient cell into the Hfr cell
- 1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps and temperatures of one PCR cycle. 2. Explain the purposes of the following components of gel electrophoresis: agarose, loading buffer, DNA ladder/marker, electrode buffer, electrodes. 3. Explain the expected results of the Alu sequence at PV92 that we tested, in terms of numbers of bands and their sizes. A labeled drawing would suffice.1. How does PFGE separate larger fragments more efficiently than standard electrophoresis? 2. Why is SYBR green less toxic than EtBr? 3. What are the similarities and differences between Manual and Automated Sanger Sequencing? 4. What is the relationship between DNA fragment length and the distance it will run in a gel? (Restriction Enzyme Digestion)1. As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance gene and a lacZ gene, within which is located a multiple cloning site. Briefly describe how one selects for/identifies bacterial cells containing recombinant pBluescript II molecules and explain how the selection/identification works.