If Johnny Joestar measured the amount of light absorbed by the analyte in a sample to determine its concentration, what kind of method did he use? Volumetric method Gravimetric method Electroanalytical method Spectroscopic method
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If Johnny Joestar measured the amount of light absorbed by the analyte in a sample to determine its concentration, what kind of method did he use?
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Volumetric method
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Gravimetric method
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Electroanalytical method
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Spectroscopic method
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Solved in 2 steps
- A sample is dissolved in water and analyzed using spectrophotometry. The sample shows an absorbance peak at a wavelength of 486 nm. Which one of the following adjustments would increase the absorption at that wavelength? O Diluting the sample with additional water. O Evaporating some of the water from the sample. Decreasing the path length, b. the light takes through the sample. Re-measuring the absorbance of the blank (water).The same water sample from the cave was subjected to an analysis to determine its nitrite concentration. Using a spectrophotometer, the following results were obtained for standard solutions of nitrite and for the unknown. Solution Absorbance at 550 nm 2.00 µM 0.065 6.00 µM 0.205 10.00 μΜ 0.338 14.00 μΜ 0.474 18.00 μΜ 0.598 Unknown 0.402 a. Draw a calibration curve base on the data from the standards. b. Find the slope and intercept of the curve. c. Determine the concentration of nitrite in the water sample.A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182x + 0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg). Determine the quantity of protein in a solution that has an absorbance of 0.344. A blank solution has an absorbance of 0.095. quantity of protein: µg
- A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A. 52 B. 3.1 C. 2.4 D. 0.52 E. 0.031You are required to measure a sample that absorbs at a wavelength of 300 nm and 4 µm.What would you use as your light source? Justify your answer.Table II Cuvette # Concentration Absorbance 0.50 M 1 1.049 2 0.40 M 0.926 3 0.20 M 0.740 0.298 4 0.10 M 5 0.05 M 0.202 Unknown # 9 0.427 Unknown # 10 7 0.646 Blank (DI water) 0 1. If Beer's Law is valid, the value of "E" should be constant. Use A = E*C*L or E= A/(E*L) and the data from Table II to calculate values of E for the five solutions below The value of L is normally 1 cm so E = A/C in this experiment. Table III Solution # Calculated Values of E 1 2 3 4 5
- The equation of your standard curve of absorbance versus concentration (mM) is y = 1.245x + 0.0012. You put a solution of unknown concentration into the Spec20 and get an absorbance value of 0.227, which lies within the standards used to make the standard curve. What is the concentration of the unknown solution in mM?How did you decide on the wavelength at which you will read the absorbance of the diluted solutions? What is the importance of reading the absorbance of diluted solutions at λmax? What will vary if you chose a wavelength 20 nm lower or higher than your chosen wavelength? (Minimum of 8 sentences)Calculate the specific absorbance ratio (SAR), where A620 = 0.104 and A280 = 1.270. Enter your result with 3 decimal places.
- The nickel level in a soil sample was determined using AAS. A 3.0 g sample was dissolved in acid and then diluted to a total volume of 100.0 mL. The absorbance of this solution determined at a wavelength of 232 nm, was found to be0.3. Several standard solutions were tested under the same conditions and a calibration curve shown below was obtained. Calculate the mass of nickel in mg.P 1. Absorbance Given the following standard curve of Absorbance vs Concentration (mM), what in the concentration of Aspirin if the absorbance is O.822? 1.2 y = 0.0032x+0.1043 R2 0.9975 0.8 0.6 0.4 0.2 250 000 350 Concentration MacBook Air 08 ODO F4 110 F8 F5 DD 93 63 6. R.Kiko wants to determine the concentration of an unknown solution which produces a green solution. However, the spectrophotometer he uses doesn't have a function to determine the analytical wavelength of the solution. What is the most probable analytical wavelength he can use for the experiment? Refer to the color wheel below.