If Jotaro Kujo measured the amount of current that passes through a sample to measure the amount of analyte in the sample, what kind of method did he use? Volumetric method Spectroscopic method Gravimetric method Electroanalytical method
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If Jotaro Kujo measured the amount of current that passes through a sample to measure the amount of analyte in the sample, what kind of method did he use?
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Volumetric method
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Spectroscopic method
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Gravimetric method
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Electroanalytical method
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- The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1What are the differences between systematic and random errors and how do they effect accuracy and precision? In what circumstances would you use standard addition (versus a normal calibration curve) to determine the amount of an analyte in a sample? A urine sample, containing analyte Z is analysed by the standard addition method where 5 mL of the original sample was mixed with increasing amounts of a Z standard and each solution diluted to a volume of 50 mL prior to analysis. A plot of the final concentration of the standard in each of the 50 mL samples (x axis) versus The measured signal from the analysis of each 50 mL sample (on y axis) produced a straight line with the general equation: y = 44.72x + 4.06 what was the final concentration of Z in the 50 mL standard addition sample? what was the initial concentration of Z in the original urine sample?What is Donnan's theory? What is its importance in biophysical chemistry?
- The protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 5.00 mg/mL BSA stock solution. To these dilutions, 100 µl of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm. Standard # BSA, in mg/mL Volume of Bradford reagent, A595 in mL 1 5.0 0.000 1.00 5.0 0.134 3 2.00 5.0 0.265 4 3.00 5.0 0.388 4.00 5.0 0.497 The supernatant dilution was prepared by mixing 49 µL of the protein isolate with 16 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.413. Note: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of calculation.What are the advantages and disadvantages of volumetric and gravimetric analysis?Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. X
- use ion exchange method in analytical separation to separate mercury and nickel from polluted water using analytical steps and protocols.Normality of a solution was determined by five different titrations. The results were 9.5 mL, 8.5 mL, 9.1 mL, 9.3 mL, and 9.1 mL. Calculate the mean, median and standard deviation of this dataWhat are the types of gravimetric methods? Give an examples where each method is used in an analysis. What is particulate gravimetry? When do we use this technique?
- 5. The color change of a chemical indicator requires an overtitration of 0.03 mL. Calculate the percent relative error if the total volume of titrant is a. 50.00 mL b. 10.00 mL 6. From the Gaussian (normal) error curve, what is the probability that a result from a population lies between 0 and +1 σ of the mean? What is the probability of a result occurring that is between +1 σ and +2σ of the mean?A water sample was analyzed for iron content using the iron-phenanthroline method. Using the set of data from Question 15 (as presented below), Reagent Blank Absorbance Absorbance (au) Reagent Blank Absorbance 0.003 Concentration of Stock Solution: 10 ppm Volume of Stock Solution (mL) Total Volume of Standard Solution (mL) 0.50 10.00 1.00 10.00 2.00 10.00 3.00 10.00 4.00 10.00 5.00 10.00 Volume of Trial Unknown Solution (mL) 1 5.00 2 5.00 3 5.00 find the true concentration of Fe2+ of the original sample. A) 5.12 ppm B) 2.56 ppm 2.52 ppm D) 5.05 ppm Concentration of Standard Solution (ppm) Total Volume of Unknown Solution (mL) 10.00 10.00 10.00 Absorbance (au) 0.012 0.132 0.267 0.349 0.428 0.560 Absorbance (au) 0.289 0.296 0.281* The data in the table below were obtained during a photometric determination of glucose in blood serum. A serum sample gave a transmittance of 0.45. Find the glucose concentration in the blood serum. * Glucose 0.0 2.0 4.0 6.0 8.0 10.0 concentration, mM Absorbance 0.002 0.15 0.294 0.434 0.5704 0.704