if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?
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if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?
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- In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.If you have an eyepiece magnification of 10x and an objective magnification of 4x, what is the total magnification? If you have an eyepiece magnification of 10x and an objective magnification of 10x, what is the total magnification?
- If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) ExplainWhat is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawGiven the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
- If a darkfield condenser causes all light rays to bypass the objective, where does the light come from that makes an object visible in a dark field?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?Using the Field of View (FoV) formula: (Low Magnification/High Magnification)= (High Field of View/Low Field of View) determine the FoV in microns for all other objective lenses (4x,10x,40,100x) given that the diameter of the 4X scanner FoV is 3.2 mm Please, explain with the formula how it was used and how values were added
