If we digested 4.5 μg of total chDNA with Sal I in a total volume of 60μL and we want to set up a 3:1 ratio using 0.1 μg of digested pGEM, how many μg of the chDNA do we ideally want and in what volume? Answers: a. 1.2 μg in 16.0 μL   b. 0.3 μg in 4.0 μL   c. 0.9 μg in 12.0 μL   d. 0.6 μg in 8.0 μL

Human Anatomy & Physiology (11th Edition)
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If we digested 4.5 μg of total chDNA with Sal I in a total volume of 60μL and we want to set up a 3:1 ratio using 0.1 μg of digested pGEM, how many μg of the chDNA do we ideally want and in what volume?

Answers: a.

1.2 μg in 16.0 μL

  b.

0.3 μg in 4.0 μL

  c.

0.9 μg in 12.0 μL

  d.

0.6 μg in 8.0 μL

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