If you have a transparent or thin specimen, do you need to open or close the iris diaphragm? As magnification decreases, does the light intensity need to increase or decrease?
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If you have a transparent or thin specimen, do you need to open or close the iris diaphragm? As magnification decreases, does the light intensity need to increase or decrease?
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- Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?While looking into your compound light microscope you find that only part of the specimen is visible. What might be the cause? Question options: Insufficient illumination from the substage condenser. Iris diaphragm has not been properly adjusted. Your objective lens has not clicked fully into position. Your objective lens is dirty.which objective lens is in place if the object you are viewing is magnified 1000x? (assume ocular lens magnification = 10x)
- While looking into your compound light microscope you are unable to properly resolve the specimen. What might be the cause? Question options: You have a dirty objective lens. Any of the choices The iris diaphragm has not been properly adjusted. The coverslip has a fingerprint on it.Which objective lens has the least working distance?Which power (low or high) will magnify the object more?
- To obtain a 400X magnification image you may choose a 40X objective lens with a 10X projector lens, or a 20X objective lens with a 20X projector lens. What are the differences in their image quality?If you have chosen the objective lens with 10x and the ocular lens magnification is also 10x. Then the total magnification will be 100x. True FalseTo alter the amount of light coming through the stage someone should adjust the: Group of answer choices 1. eye pieces 2. coarse focus 3. objective 4. iris diaphragm 5. fine focus
