In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.
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In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.
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- What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.
- Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of AcetaminophenMost of the following conclusions regarding the photo shown above are correct Except: All of the following are correct regarding the disk diffusion test photos EXCEPT : Zone of inhibition Chlorine Chlorine Chlorine O-phenylphenol Hexachlorophene O-phenylphenol Hexachlorophene Hexachlorophene O-phenylpheno Quat Quat Quat Staphylococcus aureus (gram-positive) Escherichia coli Pseudomonas aeruginosa (gram-negative) (gram-negative) Select one: O to. Gram negative bacteria are the most sensitive to chemical agents O b. Only one of the four chemicals affected Pseudomonas O c. Gram positive bacteria are the most sensitive to disinfectants O d. Hexachlorophene was effective only against Gram positive bacteria. O and. Chlorine was effective against all bacteria bacteria testedPrepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the ninhydrin reagent. What is the concentration of lysine in the final reaction mixture?
- Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computationsThe usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?
- What is the role of imidazole in Ni-Affinity Chromatography?What happens if you exclude one or more data sets of the peak area ratio of the chromatograph, how does it affect the mean concentration of the analyte and its linear requationIn a neutral solution, the [H*] is : * 1.0x10-14M zero 1.0×107M O 1.0x10-7M