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Q: Distinguish between in vivo and ex vivo gene therapy inhumans.
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Q: pick a specific disease that is currently a candidate for gene therapy—(a)briefly describing the…
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Q: What is the difference between somatic gene therapy and germ-line gene therapy?
A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…
Q: Explain why a selectable marker is frequently included in a cloning vector.
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Q: Which vector is used to replace the defective gene in gene therapy?
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Q: Explain why a cloning vector usually includes a selectable marker.
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Q: How gene therapy can be used in treating cancers? Explain broadly.
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Q: What are the two types of gene therapy?
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- In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account? Explain your answer.
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- Answer the two parts of the question. a) Explain what gene therapy involves.b) Discuss how gene therapy can be used to treat/cure or prevent a disease and include in your answer any current challenges associated with its use.Give a schematic diagram of how we can Treatment Hemophilia by using gene therapy? Please answer at your own words,please..What is the role of a vector in gene therapy?
- Make a list of ethical considerations for using gene therapy.Explain an example of a successful gene therapy trial. In your answer be sure to consider: a description of the disease condition that was treated, the mutation or disease gene affected, the therapeutic gene delivered, and the method of delivery use for the therapy.What is the advantage of using stem cells for genetherapy or gene editing?
- A patient is diagnosed with lung cancer using imaging. To establish the best treatment option, doctors ask to profile the gene expression of the tumor, using a biopsy and microarray analysis. Describe the steps involved in the analysis in detail. The analysis reveals that a new gene therapy could be beneficial to the patient. Explain briefly how gene therapy could be used to cure cancer and detail how the treatment can be formulated for delivery to the patient. To prepare DNA for the gene therapy formulation, it is often important to measure the size of the molecule. Explain how this measurement can be done using agarose gel electrophoresis, taking care to detail the property of the DNA molecule at the basis of the technique. To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on gel electrophoresis, specific sequences can be amplified by PCR. For a sequence of a 100 base pairs, calculate the number cycles of PCR required to generate 1 ng of…Why is using genomic DNA in gene therapy more efficacious?Genetic information has become part of our culture and it is difficult to tell the difference between unmodified and genetically modified food sources such as plant and animals. After reading this module’s material regarding vectors in biotechnology, consider the potential for nanotechnology and scientific advancement.Research nanotechnology and its potential use in biotechnology. In one or two paragraphs, explain the potential advantages and disadvantages of nanotechnology in health care, agriculture, or industry and discuss whether you would or would not support further research.