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In spectrophotometry, does the sample tested contain the allowable concentration of Allura red as prescribe by the FDA?
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- * The data in the table below were obtained during a photometric determination of glucose in blood serum. A serum sample gave a transmittance of 0.45. Find the glucose concentration in the blood serum. * Glucose 0.0 2.0 4.0 6.0 8.0 10.0 concentration, mM Absorbance 0.002 0.15 0.294 0.434 0.5704 0.704To run a spectrophotometry experiment, begin by warming up the spectrophotometer and preparing the samples. Be sure to select the correct absorbance (X), then run a measurement on the sample solution. Follow up by running measurements on sample solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples. Partially correct (2 of 4 correct)Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.
- given the following results " create a “standard curve” (a graph) that shows the concentration on the x-axis and the absorbance on the y-axis" and "a solution of copper(II) sulphate with an unknown concentration was found to have an absorbance of 0.09. Use your standard curve to determine its concentration" ample Number Concentration of Copper(II) Sulphate (mM) Measured Absorbance 1 0 0 2 50 0.12 3 100 0.24 4 150 0.35 5 200 0.47 Unknown 40 0.09Provide an appropriate response. 12) The IQ scores of adults are normally distributed with a mean of 100 and a standard deviation of 15. Find the probability that a randomly selected adult has an IQ score between 85 and 125.The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 0. 0.2 .063 3 0.4 .163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 = excellent data; r2 0.98-0.95 good data r2 0.94 -0.90 = fair data. How would you describe this data?- To find the relative sensitivity, for each of tubes 2-6 (neglect blank), perform the following operation absorbance (Lowry)/Absorbance (Biuret x 0.5 gram albumin (Biuret)/0.05 gram (Lowry) x 200 ml (Lowry)/100 mL (Biuret). This implifies to Relative sensitivity = 20 dilution factor x absorbance (Lowry)/Absorbance (Biuret). Report your relative sensitivity as an average of all 5 values.
- 8. Chemical Formula: C₂H₁7NOS 8 1H, broad 7 6 Final Answer 1H, broad 5 بللي بلد 4 PPM 3H s 3 2 9H s 1 there are 3 peaks between 4.00 and 4.30 ppm: a 1H t at 4.3 ppm two 1H t that both happen to be at 4.0 ppm, but are not the same 0Consider the following data set: Sample 1 2 3 4 5 Diluted Unknown (25% dilution) Diluted Unknown (50% dilution) Undiluted Unknown Absorbance at 470 nm 0.032 0.058 0.090 0.114 0.140 0.072 0.138 0.210 Concentration 1.00 x 10-³ M 2.00 x 10-³ M 3.00 x 10-³ M 4.00 x 10-³ M 5.00 x 10-³ M 1. Using MS Excel, plot a calibration curve for samples 1 to 5 (Attach a printed copy of the graph). Determine the molar absorptivity. 2. Calculate the concentration of the diluted and undiluted unknown using the linear regression equation produced from #1. 3. Back-calculate the concentration of the undiluted unknown using the calculated concentration of the 25% and 50% diluted unknown from #2. 4. Explain any significant difference that you see between the concentration of the undiluted unknown that is back-calculated from the absorbance of the 25% and 50% diluted unknown, and the concentration that is directly calculated from the absorbance of the undiluted unknown. 77Question: A pharmaceutical company wants to determine the purity of a new drug compound. The company has access to various analytical techniques such as HPLC, GC, IR, and NMR. Which analytical technique(s) would you recommend the company to use to determine the purity of the new drug compound? Why?