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A: Ion chromatography- It is used for water chemistry analysis. Ion chromatography helps in measuring…
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A: The answer of the question is given below:
Q: . What is lon chromatography?
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A: The answer is given below
Q: Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385 s,…
A: The solution of the question is given below:
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Q: Two components in an HPLC separation have retention times that differ by 22 s. The first peak elutes…
A: Soln
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A: Given tc = 209.6 sωc = 14.4 sta = 260.0 sωa = 21.2 sd = 22.1 s
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A: What are the disadvantages of uses the Thick Layer stationary phase in Thin Layer Chromatography…
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A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
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A: Temperature programming in gas chromatography is:
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A: Given observation, Silanization reduces tailing of chromatographic peaks.
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Q: See image below
A: Here we are required to find the compound present in each of the unknown mixture
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A:
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A: Length of the column = 15 cm Retention time for A = 3.5 min Retention time for B = 4.5 min
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Q: 1. Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385…
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- In TLC, why does a 3:1:1 ratio of the developing solvent of n-butanol/acetic acid/water move up the TLC plate so slowly?Explain why TLC cannot be used for some volatile low molecular weight substances such as toluene.In reversed phase HPLC, how will the retention time of a component of a mixture vary with the polarity of its molecules? Explain your answer
- A chemist was able to extract a biologically active compound and wants to purify the sample since he is sure that the sample still contains impurities. After running the extract in TLC, the chemist found out that the sample produced only one spot near the solvent front. Suggest how the chemist can achieve a better resolution for the chromatogram.what chromatography seperation how is tlc part of itUse a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
- 1. For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning.b) What are some advantages of using the Internal Standard method for quantitation over the External Standard method?c) How is the signal intensity seen in the chromatogram affected by the wavelength used for analysis?Explain the term gradient elution mode in HPLC Differentiate between gel filtration and gel permeation chromatography.Thin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!
- In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesConsider the following errors that could be made when running TLC. Indicate what should be done to correct the error. a) A two-component mixture containing 1-octene and 1, 4-dimethylbenzene gave only one spot with an Rf value of 0.95. The solvent used was hexane. b) When a TLC plate was developed, the solvent front ran off the top of the plate.If the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoning