In vitro experiments are conducted at pH = 7.4 to simulate physiological conditions. A phosphate buffer system is often used. H₂PO → H₂PO¹²¯ +H+ pK₁ = 7.2 a. What must be the ratio of the concentrations of HPO to H₂POд ions? b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4 (aq) in the preparation of the buffered solution?
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- Consider a buffer solution that contains 0.55 M NH2CH2CO2H and 0.35 M NH2CH2CO2Na. pKa(NH2CH2CO2H)=9.88. a. Calculate its pH. b. Calculate the change in pH if 0.155 g of solid NaOH is added to 250 mL of this solution. c. If the acceptable buffer range of the solution is ±0.10 pH units, calculate how many moles of H3O+ can be neutralized by 250 mL of the initial buffer.Describe the preparation of 2.00 L of 0.100 M glycine buffer, pH 9.0, from glycine and 1.00 M NaOH. What mass of glycine is required, and what volume of 1.00 NaOH is required? The appropriate pKa of glycine is 9.6Acetazolamide is a carbonic anhydrase inhibitor that is used to treat glaucoma. It has two acidic groups and has 2 pKb’s as shown below: What is the concentration of acetazolamide (B), in a 0.5000 M solution of acetazolamide (B)? What is the concentration of hydrogen acetazolamide (BH+) in a 0.5000 M solution of acetazolamide (B)?
- Predict the diffusivity of human serum albumin at 293 K in water as a dilute solution and compare it with experimental data. Assume the following: The molecular weight of human serum albumin, MA= 72,300 kg/kmol The viscosity of water at 293 K is 0.897 x 10-3 Pa∙s The experimental data value is 5.93 x 10-11 m2/s.Acetazolamide is a carbonic anhydrase inhibitor that is used to treat glaucoma. It has two acidic groups and has 2 pKb’s as shown below: What is the concentration of dihydrogen acetazolamide (BH22+) in a 0.5000 M solution of acetazolamide (B)? What is the concentration of hydronium ion (H+) in a 0.5000 M solution of acetazolamide (B)?Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of the original suspension if the thousand-fold dilution of that suspension has an absorbance of 0.3 at 600nm? * A. 3.7 x 10^12 B. 3.7 x 10^9 C. 3.7 x 10^1 D. None of these is correct
- At 39.9ºC, a solution of ethanol (XetOH = 0.9006, P * etOH = 130.4 Torr) and isooctane (P * iso = 43.9 Torr) forms a vapor phase with YetOH = 0.6667. The total pressure is 185.9 a. Calculate the activity and the activity coefficient of each component.b. Calculate the total pressure the solution would have if it were ideal.c. Comparing the ideal pressure to the actual pressure, what does this indicate about the molecular interactions?You have been provided with stock solutions of: stock A: 0.06 M sodium pyrophosphate buffer pH 8.5 stock B: 3 M ethanol stock C: 0.015 M NAD+ stock D: milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.Calculate the following concentration given this
- For an acid HA, the concentrations of HA and A are 0.075 and 0.025, respectively, at pH 6.0. What is the p K a value for HA?An unknown mixture is known to contain only Ba(OH)2 (MW=171.34 g/mole) and NaOH (MW=40.0 g/mole). If the mixture is known to contain 45% by mass NaOH, and 8.0 grams of the mixture is dissolved completely in 50.0 ml of solution, answer the following. c).If 10.0 ml of a 0.2 M solution of Na2SO4 was added to the 50.0 ml solution, what would be the final concentration of Na+ in solution.In an experiment, what happens to the calculated molar mass after waiting an hour between thawing the frozen cyclohexane and adding the unknown compound, during which time some of the cyclohexane evaporated. Will the molar mass be too large, too small, or unaffected? Explain.|
