In which of the three types of co-IP, the antibody elutes with the protein of interest and its heavy and light chains show up on a gel? Group of answer choices Classical Crosslinking Direct All of the above

In which of the three types of co-IP, the antibody elutes with the protein of interest and its heavy and light chains show up on a gel? Group of answer choices Classical Crosslinking Direct All of the above

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter17: Genes And The Immune System

Section: Chapter Questions

Problem 12QP: The Adaptive Immune Response Is a Specific Defense Against Infection Describe the genetic basis of...

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Concept explainers

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.

Question

In which of the three types of co-IP, the antibody elutes with the protein of interest and its heavy and light chains show up on a gel?

Group of answer choices

Classical

Crosslinking

Direct

All of the above

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Antibody heavy and light chain polypeptides consist of repeated domains, each of which is ~110 amino acids and folds up into a compact three-dimensional structure known as an ‘immunoglobulin domain.’ These immunoglobulin domains are: Mixed and matched between different antibody heavy and light chains to produce variability Always identical to each other within a single antibody heavy chain or light chain polypeptide Always differ in amino acid sequence between different light chain polypeptides in both of the two light chain immunoglobulin domains Similar but not identical in amino acid sequence when comparing the domains in a single heavy chain polypeptide Identical in amino acid sequence for every domain when comparing different antibody heavy chain polypeptides to each other
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hinge region Fc Fab kappa alpha chain Drag answer here Drag answer here Drag answer here Drag answer here Drag answer here provides flexibility to permit binding to different antigenic arrangements a heavy-chain isotype a light-chain isotype the arms of the antibody that bind antigen the stem that carries out effector function of antibodies through interaction with cell-bound receptors and serum proteins
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