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- Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture. What is the first step to prepare your Kirby-Bauer test?Would you expect the results from the MAC test to corroborate the results from the EMB test? Explain why or why not.
- Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry methodDiscuss the significance of the following processes and what constituent is being determined: a. Borntragers’s test b. Bate-Smith and Metcalf method c. Wilstatter “cyanidin” testYou made your material in the lab and wish to test for biocompatibility in vitro with a direct contact test. Describe your experimental procedure, include your experimental protocol, any controls you would include, and expected results if the material is biocompatible.
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