Match each microscope component with its description or function. 1. Objective 2. Ocular 3. Condenser 4. Diaphragm a. gathers light rays and focuses on object b. eyepiece c. magnifying element located on nosepiece d. disk with an opening that adjusts amount of light that contacts specimen
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- 1. Match: Read about each microscope part. Match the description to the part on the diagram. Stage: Platform where a slide is placed. Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly magnifies a sample by 10x. Coarse focus knob: Large knob that moves the stage up and down to focus the sample. Fine focus knob: Small knob that moves the stage over a short distance to refine the focus. Objective lens: A second lens that further magnifies the sample. Microscopes usually have several objective lenses with different magnifications. Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.2. What is the function of a revolvillg 3. Which connects the eyepiece to the revolving nosepiece with the objectives? 4. Describe the function of the mirror. 5 What are parts of the microscope are being connected by the inclination joint? What I Have Learned Directions: Fill in the blank to make the sentences complete. Choose the correct words listed in the box. is a curved glass that bends light that passes through it. regulates the amount of light on the specimen. 1, 2. for viewing. 3. The glass slide is placed on the 4. The bottom of the microscope, used for support is 5. The 6. 7. To transfer a microscope, use the holds two or more objective lenses and can be rotated to easily change power. is the lens at the top of the microscope that you look through. to carry the microscope. 8. is a small knob on the side of the microscope used to fine-tune the focus of the specimen. 9. The arm and base of themicroscope are being connected by the 10. The is used to enlarge the specimen. stage…
- Which of the follow are correct statements about microscope handling and care? 1. Always use TWO HANDS to carry the microscope. 2. Only use LENS PAPER to clean the lenses. Never wipe back and forth, but simply make a gentle swipe across the lens. 3. A soft cloth should be used to clean the lenses. 4. Start and finish the day’s work at the LOWEST MAGNIFICATION. 5. Start and finish the day’s work at the HIGHEST MAGNIFICATION 6. Always BEGIN examining a specimen with a LOW POWER objective. 7. Always BEGIN examining a specimen with a HIGHEST POWER objective 8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective. 9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective. 10. Unplug the microscope by pulling on the plug, not the cord.Match the part of the microscope with its use. Dragged and dropped options on the right-hand side will be automatically saved. For keyboard navigation... SHOW MORE Stage Nosepiece Vertical Stage Knobs Course and Fine Adjustment Knobs Iris Diaphram III ||| Allows for changing objective lenses Move the stage side to side Hold the slide in place Control light passing through the stage Move the stage up and downWrite the term that the phrase describes. 1. Large knob that moves the stage or objective lens a great distance. Used with scanning or low-power objective lenses only. 2. Flat platform beneath the objective lens on which the microscope slide is placed. 3. Removable lenses that you look through to observe the microscope slide. 4. Small knob that moves the stage or objective lens a very small distance and is used for precision focusing. 5. Extends from the arm and contains the ocular lenses and rotating nosepiece. 6. Lens that condenses light through the specimen and is located below the stage. 7. Light from specimen passes through these lenses first. These lenses are located in the rotating nosepiece. 8. Wide bottom part that supports the microscope. 9. Regulates the amount of light passing through the condenser. 10. Vertical portion that connects the base to the head.
- B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. Base8. If the ocular lens of a microscope has 10x magnification, calculate total magnification: Objectives Ocular lens Total magnification 10X low power Click/tap to enter Click/tap to enter 40X high dry Click/tap to enter Click/tap to enter 100X oil-immersion Click/tap to enter Click/tap to enterA. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Power
- 5) Label the parts of the microscope. 4 1 5 7 8 Nikon 10 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. COWhich of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)
