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- In this image a stage micrometer and an ocular micrometer are aligned so that the 10 o.u. line aligns with the 100um line. The 200um line of the stage micrometer overlaps with the 90 o.u. line of the ocular micrometer. What is the calibration for this lens? eyepiece graticule 40 50 60 70 s0 9 100 stage micrometer 0.8 um/ou O 8 um/ou O 1.25 um/ou 0.25 um/ouContrast for a specimen may be adjusted by _____. Question options: Adjusting the iris diaphragm Lowering the stage Turning off the substage condenser light source Changing the objective lensPreparing a wet mount of the letter "e”. 1. Obtain a prepared ‘e’ slide. 2. Focus the slide first with the scanning objective, then click to lower power and focus again. 3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fine adjustment knob. 4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification. The LPO, HPO, and Oil Immersion
- Preparing a wet mount of the letter "e”.1. Obtain a prepared ‘e’ slide.2. Focus the slide first with the scanning objective, then click to lower power and focus again.3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fineadjustment knob.4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification of Oil Immersion objectiveCompute the total magnification. [Use 10x magnification for eyepiece]1. Scanning2. LPO3. HPO4. OIOWhat is the DFV of a 40x objective lens if the DFV of a 10x objective lens is 3 mm? Express your answer in μ (micra)
- Diseussion: What is the microexamination, why it is used and what is the difference between macro and microexamination? 2. state the purpose of the followings: Coarse adjustment knob, b. Fine adjustment knob, e. Iris diaphragm. d. Objective lens. e. Ocuiar or eye lens. Lengith, e 3. If the approximate magnification when using an objective lens with a focal Y re length of (2mm) is (800X), what is the magnification of the eye lens. 4. Calculate the focal length of the objective lens for a microscope with an approximate magnification of (1000x) and an eye lens magnification of (10x). * 5. Explain briefly the microscope work mechanism to examine a specimen prépared for microexamination.Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light source8.Explain what the coarse focus knob does and what the fine focus knob does. 9. What is different between the 100X objective and the other objective lenses? In other words, what does the 100X need in order to use it? please give answer for both please
- The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.To alter the amount of light coming through the stage someone should adjust the: Group of answer choices 1. eye pieces 2. coarse focus 3. objective 4. iris diaphragm 5. fine focus1. Calculate the total magnification for the following: A. Scanning power objective = 5x; eyepiece =4x B. High power objective = 40x; eyepiece= 5x c. Low power objective = 10x; eyepiece= 10x