mine whether each event occurs during initiation, elongation, or termination. Initiation The first AUG codon is recognized. In E. coli, mRNA binds to the 30S ribosomal subunit. Elongation In E. coli, EF-G-GTP displaces the tRNA in the A site. In E. coli, EF-Tu hydrolyzes GTP. An aminoacyl-tRNA enters the A site. Termination In E. coli, an initiation factor blocks the A site. The ribosome has mRNA, an empty A site, and a deacylated tRNA in the P site.

mine whether each event occurs during initiation, elongation, or termination. Initiation The first AUG codon is recognized. In E. coli, mRNA binds to the 30S ribosomal subunit. Elongation In E. coli, EF-G-GTP displaces the tRNA in the A site. In E. coli, EF-Tu hydrolyzes GTP. An aminoacyl-tRNA enters the A site. Termination In E. coli, an initiation factor blocks the A site. The ribosome has mRNA, an empty A site, and a deacylated tRNA in the P site.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 16QP: Given the following tRNA anticodon sequence, derive the mRNA and the DNA template strand. Also,...

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How does the antibiotic streptomycin inhibit bacterial translation? Multiple Choice blocks elongation by preventing the large ribosomal subunit from binding to the small ribosomal subunit interferes with the normal pairing of aminoacyl TRNAS and codons resulting in abnormal proteins prevents the release of the initiator tRNA from the P site, blocking elongation blocks termination by competitively inhibiting the binding of a release factor to the A site
Determine whether each event occurs during initiation, elongation, or termination. Initiation Initiator tRNA enters the P site. Elongation Answer Bank In E. coli, EF-Tu delivers an aminoacyl-tRNA to the ribosome. In prokaryotes, the Shine-Dalgarno sequence pairs with rRNA. The ribosome has mRNA, an empty A site, and a deacylated tRNA in the P site. IF2 dissociates. In E. coli, EF-Tu hydrolyzes GTP. Termination Translocation occurs.
identify start/end site, which amino acid will be on the tRNA that is the first to bind to the A site of ribosome, anticodon on the tRNA in the P site of the ribosome when release factor bings to A site, and what amino acid sequence of the protein that will be formed from mRNA? Here is the mRNA sequence:5'GUUUCCCGUAUACAUGCGUGCCGGGGGCCCGUUACCAGGCCUCAUUAUUGGAUAACGGAAAAAAAAAAAAA3'
Using the following list of codons describe, using diagrams etc., how information stored in the DNA is translated into a peptide. Be sure to discuss all steps. In other words, use a diagram and give me sequences, transcription and translation steps. Show the sequences of the sense and the other DNA strand, the mRNA and the tRNA’s. UUU -phenylalanine UCU -serine AUG –initiation/methionine CUU -leucine ACU -threonine GUU -valine UAA -Termination
E channel Charged tRNA Anticodon for START Initiating factors Release factors m III III exit site for an uncharged stem of tRNA UAC located on tRNA's anticodon arm proteins that help mRNA bind to a small and large ribosomal subunit to start translation a tRNA that contains its appropriate amino acid attached to the acceptor arm bind to STOP codons and result in a hydrolysis reaction that creates a drop of water that halts translation
Which one is the next tRNA that will arrive to the "A" site of this ribosome? Gly Previous Arg Glu Pro Val OCys U A G G Met Pro U A G G
Iove aIung the HRNA from codon to codon, the appropriate amino acids are brought into place and linked together according to the sequence of codons. Thus, the code in the mRNA is translated into a special sequence of amino acids. The order of the amino acids in the protein, therefore, is specified by the MRNA, which in turn is transcribed from the DNA. Procedures and Observations: During transcription, the DNA double helix unwinds and "unzips. The two strands separate as the hydrogen bonds binding the nitrogen bases break. Then, nucleotides present in the cell line up along one strand of the DNA, the order of the nucleotides determined by the order of the nucleotides in the DNA. As the mRNA forms, uracil (U) nucleotides match with adenine (A) nucleotides; cytosine (C) nucleotides match with guanine (G) nucleotides. Note: RNA contains uracil (U) nucleotides where thymine (T) nucleotides would occur in DNA. The nucleotides in the newly formed mRNA are complementary to the nucleotides of…
Determine whether each event occurs during initiation, elongation, or termination. Initiation Peptidyl transferase transfers the peptidyl group to water. In E. coli, EF-Tu hydrolyzes GTP. Elongation In E. coli, mRNA binds to the 30S ribosomal subunit. Answer Bank In prokaryotes, the Shine-Dalgarno sequence pairs with rRNA. In E. coli, EF-Tu delivers an aminoacyl-tRNA to the ribosome. Termination Translocation occurs. Initiator tRNA enters the P site.
Which of the triplets below is a possible anticodon for a tRNA that transports Leucine (Leu) to a ribosome? Second letter C UGU cys UCU1 UCC UCA UCG UAU Tyr UACS Ser UAA Stop UGA Stop A UAG Stop UGG Trp UUU UGCS Phe UUC U UUA UUG FLeu CAU HiS CGU] CUU CUC Leu CCU ССС CCA CCGJ CACS CGC Arg CGA CAA GIn Pro C CUA CUG J CAG S CGG AUU AUC lle AUA AAU Asn AGU ser ACU АСС Thr ACA AAC AAA ys AGA Arg AAG J AGC AUG Met ACG Lys AGG J GUU GUC CUA Val GAU ASP GCU GACJ GCC FAla GGU] GGC CGA Gly CCA C CAA M UCAG Third letter UCAG UCAG First letter
Which of the following events would NOT result in ribosomes stalling on an MRNA during translation? The MRNA in a given region is very highly folded, and the ribosome stalls because the RNA structure blocks its movement. O The correct tRNA is scarce because it is not transcribed very much in the prevailing conditions. The ribosome stalls while waiting for one of the scarce tRNAS to diffuse into the A site. O the release tactor enters the A site, decodes a stop codon and transfers an oxxYgen from water to the carboxyl group of the peptidyl-tRNA in the P site, causing stalling O Starvation for amino acids will reduce the amount of amino-acylated (charged) tRNA the ribosome will stall while waiting for a charged tRNA to enter the A site to decode the next codon
Drag the functions involved in the bacterial translation process into the appropriate box with the corresponding name of the translation factor or component. Reset Help Prevents premature association of the large ribosomal subunit with the small Serves as the MRNA binding site for Guides the initiator fMet-tRNA into the the small ribosomal subunit. P site. ribosomal subunit. Prevents entry of an amino-acyl tRNA into the ribosome A site during the early initiation stages. Assists incoming amino-acyl RNA into the A site. IF1 IF2 IF3 EF-Tu Shine-Dalgarno sequence
What about the ribosome is NOT true? O They are large tRNA-protein complexes. It is composed of a large and small subunit. IF2 binds the fMet TRNA in the P site. O Initiation factor 2 (IF2) with GTP bound binds the AUG start codon.