Molecular mass (kd) 145- 102- 75- 43- 27- [DTT] (MM) 10.00 1 2 3 4 5 6 Samples Questions 1. How many polypeptides are present in C3c? Determine their molecular masses from the figure. What was the purpose of using DTT in this experiment? What kind of bonds hold the polypeptides 2. 3. together? 4. How is the 102 kd polypeptide related to the other polypeptide species?
Molecular mass (kd) 145- 102- 75- 43- 27- [DTT] (MM) 10.00 1 2 3 4 5 6 Samples Questions 1. How many polypeptides are present in C3c? Determine their molecular masses from the figure. What was the purpose of using DTT in this experiment? What kind of bonds hold the polypeptides 2. 3. together? 4. How is the 102 kd polypeptide related to the other polypeptide species?
Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Question
![Experiment
This experiment was designed to analyze the structure of a component (C3c protein) of the
complement system, which is involved in the immune response against microorganisms. Purified
C3c protein (molecular mass: 145 kd) was incubated in the presence of various concentrations of
the reducing agent dithiothreitol (DTT) and then subjected to electrophoresis in a sodium
dodecyl sulfate (SDS)-polyacrylamide gel. SDS disrupts noncovalent bonds and polypeptides
are separated by size during electrophoresis. The gel was stained with Coomassie Brilliant Blue,
a protein dye. The figure shows the molecular masses of intermediates and products generated by
DTT treatment.
Results:
Molecular
mass (kd)
145-
102-
75-
43-
27-
[DTT] (MM)
10.00
1 2 3 4 5 6 Samples
Questions
1.
How many polypeptides are present in C3c?
Determine their molecular masses from the
figure.
What was the purpose of using DTT in this
experiment?
What kind of bonds hold the polypeptides
2.
3.
together?
4. How is the 102 kd polypeptide related to the
other polypeptide species?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbe21ad11-6e18-4b63-a679-8109648f0f0b%2Fef754fcc-ad9e-482e-8548-9606553592a8%2F4mbszi_processed.png&w=3840&q=75)
Transcribed Image Text:Experiment
This experiment was designed to analyze the structure of a component (C3c protein) of the
complement system, which is involved in the immune response against microorganisms. Purified
C3c protein (molecular mass: 145 kd) was incubated in the presence of various concentrations of
the reducing agent dithiothreitol (DTT) and then subjected to electrophoresis in a sodium
dodecyl sulfate (SDS)-polyacrylamide gel. SDS disrupts noncovalent bonds and polypeptides
are separated by size during electrophoresis. The gel was stained with Coomassie Brilliant Blue,
a protein dye. The figure shows the molecular masses of intermediates and products generated by
DTT treatment.
Results:
Molecular
mass (kd)
145-
102-
75-
43-
27-
[DTT] (MM)
10.00
1 2 3 4 5 6 Samples
Questions
1.
How many polypeptides are present in C3c?
Determine their molecular masses from the
figure.
What was the purpose of using DTT in this
experiment?
What kind of bonds hold the polypeptides
2.
3.
together?
4. How is the 102 kd polypeptide related to the
other polypeptide species?
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