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In not more than five lines, outline a method for preparation of tomato cell suspension culture and subculture.
1. Callus: Plant callus in simple terms is a growing mass of unorganized parenchyma plant cells that cover a plant wound.
2. Cell suspension culture : Cell Suspension culture is different form of culture in which single cells or very small aggregates of cells multiply while they are suspended in agitated liquid medium for specific amount of time.
3. Subculture : A subculture is defined by transferring some or all cells from a previous old saturated culture to fresh growth medium and hence used to prolong the life and/or expand the number of cells or microorganisms in the culture.
4.Media:The best regeneration medium is the Murashige and Skoog (MS) medium for tomato cell growth.
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- Outline a method for preparation of tomato cell suspension culture and subcultureIn what instances would the pour plate method be more appropriate than the spread plat method?In paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using chromatography and electrophoresis? Please be in full details.
- kindly do a research review of related study about alcohol-based hand sanitizer with an extract from calamansi rinds, focusing on its antibacterial properties. also, strictly follow to the rrs format (using words such as 'according to', 'in a study entitled', and etc.) and cite the reference.1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)In paragraph form (about 2-3 long paragraphs), discuss how to isolate and purify antimicrobial agents from root samples. Preferably using an extraction technique. Please discuss everything in detail and provide the DOI of the journal you sourced so I can cross-check it.
- What is the purpose of this subculture procedure? In general, how is this carried out in tissue culture maintenance?What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.
- Describe characteristics of Proteus Vulgaris in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient AgarWrite an introduction related to culture media and isolation of pure culture technique (streak plate and spread plate method)In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold