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- A Photo credit: BSP3D- Group 5 Pharm. Bio. Sci 3 Laboratory (S.Y. 2016-2017) Sample is Bacillus subtilis; A-Ciprofloxacin; B-Ampicillin; C-Tetracycline; D- Vancomycin Refer to the image above and answer the following questions: 1. By just looking at the example above, which do you think is the most effective antibiotic for Bacillus subtilis? which is the least effective? and why? 2. How will you measure the zone of inhibition of vancomycin (letter D)? 3. What is the importance of evenly distributing the microbe onto the plate? 3. SubtilisGeneral Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference. Causative Agent and Disease Profile for S. aureus Template and Example ITEM MSM PROFILE MICROBIAL PROFILE I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY G MICROSCOPIC IMAGE II DISEASE PROFILE Scalded skin syndrome A DISEASE/S Skin and Wound Infections Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…IN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?
- diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14Antimicrobial agent Disk code zone of inhibition interpretation Ampicillin AM carbenicillin erythromycin gentamycin Nalidixic acidPlease describe in detail what would be a suitable procedure should you break anErlenmeyer flask containing 50 mL of a biosafety level 2 microorganism inside amicrobiological safety cabinet.
- Item 1. Describe the BEST method of sterilization or treatment to reduce pathogens to safe level for each of these 5 items. 2. Explain why this is the best method and why it is BETTER than other possible methods. (Specific treatments were mentioned in lecture for each item.) Plastic milk containers 1. Preserving low-acid vegetables to kill Clostridium botulinum endospores. 2. Liquids contaminated with prions (list BOTH methods of sterilization discussed in lecture) 3. Skin 4. Media with heat sensitive nutrients 5.what do the colors in the kirby bauer plate represent black/dark area = tan/yellow are small white circles Match choices - - source of the chemical being tested for antimicrobial activity - locations where the bacteria was able to grow - bacterial innoclunum being tested for sensitivity - locations where the bacteria was not able to growTopic: Preparation of Microbial Suspension 5. Over-dilution with sterile water. Effect: Rationale:
- 6 2 4 3 5 LABORATORY REPORT FORM EXERCISE 25 ASSAY OF ANTIMICROBIAL AGENTS: DISK-DIFFUSION METHODS What is the purpose of this exercise? Evaluate 1. For each of the disinfectants or antiseptics tested, determine the active ingredient. What class of inhibitory agent does it belong to and what is its mode of action? CHEMICAL AGENT Lysol Sanitizer Foaming Handwash Detergent. Desinfecting Hand Sanitizer ACTIVE INGREDIENT(S) Alhyl Dinethylaenzy/a. chloroxylenol 0.3% Alkyl, Dimethyl benzyl ammonium chlorides ethy benzyl A. C. Ethyl Alcohol 70% 1/Cleansing s Pray Hydrogen Peroxide Benzalkonium Lidocaine HC) An Septic CLASS OF AGENT MODE OF ACTIONG When is it appropriate for gloves X + rface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod... 2 Exit rientation to Infection Prevention Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG INTopic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:
