One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts with nasopharyngeal or oropharyngeal swabbing to get a sample. Explain concisely how this technique generally works and how it can be used for testing.
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- Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR). Which of the tests had the highest accuracy and what methodology and techniques is used for Multiplex PCR test.Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? ExplainDiscuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.
- A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 8 plaques on the 10-7 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. d.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.What are the advantages and disadvantages of Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction?
- A) One assay format that might be appropriate for developing a point of care diagnostic kit to determine the presence of a pathogen in a patient. B) One assay that would be appropriate to study which location of the lung is being targeted by a virus.list the advantages and disadvantafes of latex agglutinaton(LA) and gelatin particle agglutination tests (GPAT) techniquesAs everyone is aware of, suspected COVID-19 patients are being diagnosed using the Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction. Explain its principle; its advantages and disadvantages.
- Based on the available data, describe an appropriate and most popular method for viral detection (quick and reliable) that could be adopted at the microbiology laboratory for detection of COVID19 infection. Highlight the link between method reliability and principles involved. In addition, limitation of this approach should also be emphasized .A MLS supervisor is writing an Standard Operating Procedure (SOP) for running a PC test for Hepatitis B DNA. There are several things she must include in the instructions to run the test.1. On average, how many PCR cycles must be run to see if the test is positive or negative for HBV?2. What would a positive test and a negative test look like?3. Her younger sister wants to know what she did at work today. How should she explain what happens during each step of the PCR cycle.what are some advantages to using latex particles instead of blood cells for IM assay?