One microgram of a pure enzyme (MW = 92,000) catalyzed a reaction at a rate of 0.50 μmoles/min under optimum conditions. Calculate (a) the specific activity of the enzyme in terms of units/mg protein and units/mole, and (b) the turnover number.

One microgram of a pure enzyme (MW = 92,000) catalyzed a reaction at a rate of 0.50 μmoles/min under optimum conditions. Calculate (a) the specific activity of the enzyme in terms of units/mg protein and units/mole, and (b) the turnover number.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter30: Kinetic Methods Of Analysis

Section: Chapter Questions

Problem 30.15QAP

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Question

One microgram of a pure enzyme (MW = 92,000) catalyzed a reaction at a rate
of 0.50 μmoles/min under optimum conditions. Calculate (a) the specific
activity of the enzyme in terms of units/mg protein and units/mole, and (b) the
turnover number.

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