ou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture at 8 am on Monday with 5 x 10^5 cells. Assume all survive, a generation time of 18 hours and all cells are actively dividing. When would you expect them to be confluent? A) 7:24 AM on Saturday B) 8 pm on Tuesday C) 8 am on Tuesday D) 9 pm on Thursday E) 12:48 pm on Wednesday F) They won't grow that long
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You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells.
Question: You seed a culture at 8 am on Monday with 5 x 10^5 cells. Assume all survive, a generation time of 18 hours and all cells are actively dividing. When would you expect them to be confluent?
A) 7:24 AM on Saturday
B) 8 pm on Tuesday
C) 8 am on Tuesday
D) 9 pm on Thursday
E) 12:48 pm on Wednesday
F) They won't grow that long
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- You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells. 2) You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 105 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand? A) 1.64 x 10^5 cells B) 4.56 x 10^3 cells C) 5.23 x 10^10 cells D) 1.75 x 10^8 cells E) None of theseYou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 10^5 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand? A) 1.64 x 10^5 cells B) 4.56 x 10^3 cells C) 5.23 x 10^10 cells D) 1.75 x 10^8 cells E) None of theseYou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts ) If 20% of a culture of human cells have a DNA content somewhere between 1&2*s (S-phase=8 hours) and 1X amount of DNA in non-dividing cells. What is the generation time?
- You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts 3)You seed another culture of a different end cell line, john12 cells at 8am on Friday with 104 cells. By Thursday at noon (12:00pm) they reach confluence. Assume the same confluency as stated above for T150 flask. What is the generation time (doubling time)In Figure 10-25, why do only plant cells that have T-DNAinserts in their chromosomes grow on the agar plates?Do all of the cells of a transgenic plant grown from oneclump of cells contain T-DNA? Justify your answerFollowing is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…
- Before JCVI-syn3.0 was produced, what steps were used to create a synthetic Mycoplasma cell? Put the events below into the correct order. Drag and drop the events into the proper sequence from left to right. ▸ View Available Hint(s) First Step Transformed cells are detected by the presence of lacz, which causes them to cleave Xgal and produce a blue color. Yeast is transformed using Mycoplasma mycoides fragments. The synthetic chromosome is transformed into Mycoplasma capricolum. The synthetic Mycoplasma mycoides chromosome is purified. DNA fragments assemble through homologous recombination in yeast. Reset Help Last StepWhich of the following are involved in microtubule stabilization or growth? Choose all that apply Side-binding MAPs CLASP Op18/stathmin Tau Kinesin-13 XMAP215 EB-1 << PreviousYou seed another culture of different end cell line, Walker12 cells, at 8 am on Friday with 10^4 cells. By Thursday at noon (12:00 pm) they reach confluence. Assume the sae confluency as stated above for a T150 flask (culture area of 150cm^2 adn when confluent contains 2 x 10^7 cells). What is the generatoin time (doubling time)? A. 120 hours B. 80 hours C. 38 hours D. 8 hours E. 11.4 hours F. other ________
- A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following: What are two possible mistakes made that explains why there are so few cells? Explain why those mistakes had the effect they did.The function of FtsZ was determined in part by using a temperature-sensitive ftsZ mutant. What is the phenotype of an ftsZ mutant when it is grown at 37°C? Cells of random sizes (some very small) are formed after cell division Normal sized cells are formed after cell division Cells do not divide, and turn into long filaments Cells die immediately upon shifting to 42°CFor most cell types, serum must be added to the media to get cells to grow and proliferate in culture. Nevertheless, serum is very expensive and can complicate experiments utilizing the cells. Many companies have developed "artificial serum" which enables cells to multiply without the use of serum. See data below. What are likely components of the artificial serum that enables cells to grow. Explain (limit 3-4sentences). Non 2% Human plasma 1% Artificial serum(XF) 1% Artificial serum(AF) 104 cells/cm² Cell density cultured after 4 days 127 10- 2 0 NON 2% 1% 1% Human Artificial Artificial plasma serum(XF) serum(AF) PDL [times] 30- 25+ 20- 15- 10- 5- 0 -2% Human plasma 0 Subculture 1% Artificial serum (XF) 1% Artificial serum(AF) 5 10 15 20 25 30 Culture days Medium: HFDM-1 (+) was used.