Q.No.6 Northern blotting is used to determine the amount and size of a particular RNA transcribed in a given cell type. Alternative splicing produces mRNAs of different lengths of the same gene. In the following figure three probes were used: PSAT, PHGDH and Actin (house keeping gene). Discuss the results in detail. a. Which tissue showed higher expression? b. Why actin is used? The figure below shows Northern blet analysis of dfferent human tissues. Three probes were used: PSAT, PHGDH and Actin. Actin was used as a loading control, meaning transcniption of actin is consistent between human tissues. Which tissue showed the highest level of transcription of the PSAT gene? Northern blot results PSAT RNA PHGDH RNA Aactin mRNA

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.