Q3: The Figure shown a HPLC chromatogram of two compound.Calculate the following values using the data in this chromatogram. Detector signal a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 0.6- 05- 04 03- 02- 0.1 15 30 45.0 s void 45 60 76 كليل الك 90 1800 s uracil 247.5s guanine 105 120 135 150 166 180 195 210 225 240 255 270 296 Time, seconds
Q3: The Figure shown a HPLC chromatogram of two compound.Calculate the following values using the data in this chromatogram. Detector signal a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 0.6- 05- 04 03- 02- 0.1 15 30 45.0 s void 45 60 76 كليل الك 90 1800 s uracil 247.5s guanine 105 120 135 150 166 180 195 210 225 240 255 270 296 Time, seconds
Chapter8: Sampling, Standardization, And Calibration
Section: Chapter Questions
Problem 8.16QAP
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