Question 2: Calculate the Mid-Parent, Better Parent and Economic heterosis for plant height from the following data: Replication 2 Replication 3 Replication 1 (average height of 10 (average height of 10 (average height of 10 plants in cms) plants in cms) plants in cms) Parent 1 Parent 2 101 152 F₁ Hybrid 210 Check variety 202 101 141 205 204 98 150 208 199 Replication 4 (average height of 10 plants in cms) 101 151 210 200
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- Question 2: Calculate the Mid-Parent, Better Parent and Economic heterosis for plant height from the following data: Replication 1 (average height of 10 plants in cms) Replication 2 (average height of 10 plants in cms) Replication 3 (average height of 10 plants in cms) Replication 4 (average height of 10 plants in cms) Parent 1 100 102 99 101 Parent 2 150 148 152 151 Fi Hybrid Check variety 210 209 208 212 200 201 199 2005' 3' For numbers 6 to 10, refer to the image above and answer the questions. 6. Which among the two strands will have a continuous replication? 7. Which is the lagging strand? 8. Along which strand will Okazaki fragments appear? 9. How are Okazaki fragments joined together? 10. Where should the 3' end of the lagging strand be located? On the right or left side? inSummary table of data for one yeast DNA microarray. Shows 14 of the 6,200 genes on the full microarray. Also contained in the data is the location of each spot on the microarray. Block Column Row Red 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Gene Name tubl tub2 sec1 sec2 sec3 act1 act2 fus 1 idp2 idp1 idh1 idh2 erd1 erd2 2,345 3,589 4,109 1,500 1,246 1,937 2,561 2,962 3,585 2,796 2,170 1,896 1,023 1,698 Green 2,467 2,158 1,469 3,589 1,258 2,104 1,562 3,012 1,209 1,005 4,245 2,996 3,354 2,896 Red: Green Ratio 0.95 1.66 2.80 0.42 0.99 0.92 1.64 0.98 2.97 2.78 0.51 0.63 0.31 0.59 Induced or Repressed? In the table above, write down if the gene is induced, repressed, or equally expressed.
- These are written as either accurate or contain errors. Rewrite each one with an error as an accurate statement. Please have an explanation. Thank you! Over time telomeres shorten over time because when the primer is removed at the end of chromosome strand cannot be filled in because there is not free 3’ OH. Semiconservative DNA replication indicates that daughter strands are hybrid molecules with half (one strand being parental) and the other being newly synthesized. A mutation in the gene responsible for transcribing primase would affect only the lagging strand of DNA. In eukaryotes RNA polymerase binds to the activator, specifically at the TATA box to align with the translational start site. Transcription Factors can have more than one function domain. One is the DNA-binding domain and the other is a trans-activation domain. Additive alleles function at one gene to contribute to the phenotype of an organisms, while non-additive alleles at that one gene do not add to the phenotype.…Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA Polymerase I Primase [Choose ] Replicates bacterial chromosomes Separates DNA strands Changes Supercoiling Has a 5-to-3 exonuclease activity RNA synthesis Changes Supercoiling Has a 5-to-3 exonuclease ac RNA synthesisDetailed study of the monster Lochnessius coolnameii revealed the following: Characteristic Result Size of nuclear genome 2.04 m of DNA (B-form) Length of mitotic S phase 5 hr Rate of DNA synthesis 2,500 basepairs/min at each replication fork What is the minimum number of origins of replication needed in each cell of this monster?
- Below is a picture of a single origin of replication in a eukaryotic cell. 5' 3' 5' 1. On the figure above, Draw out where the following molecules will be located: Helicase; Sliding Clamp, Single Strand Binding Protein. 2. On the right hand side of the dotted line, the replication of which template strand (top or bottom) will be continuous by DNA polymerase? 3. On the left hand side of the dotted line, the complete replication of which template strand (top or bottom) will be more affected by a mutation that causes DNA ligase to be partially functional?1c) During DNA replication, both positive and negative supercoiling is introduced in the DNA being replicated. Name the enzymes that introduce supercoiling into DNA during replication; please clearly indicate which enzyme(s) introduce positive supercoiling and which enzyme(s) introduce negative supercoiling.Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
- 1/V (uM x min-1)-1 1. To the right is a Lineweaver-Burk plot for an enzyme that can cleave both DNA (D) and RNA (R). 2200 2000 1800 a. What is the KM when cleaving DNA? I 1/V R 1600 • 1/V D b. What is the KM when cleaving RNA? 1400 1200 C. What is the Vmax when cleaving DNA? Z 1000 800 d. What is the Vmax when cleaving RNA? 600 e. On the graph, draw an appropriate line for a 400 noncompetitive inhibitor for cleaving DNA. Label clearly. f. On the graph, draw an appropriate line for an uncompetitive inhibitor for cleaving RNA. Label clearly. 200 8 10 11 1/S (µM)-1Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?Below is a list of "solutions" to problems encountered when bacteria replicate DNA. Match the "solution" to the specific "problem" encountered during DNA replication ligase Choose] [Choose ] helicase conversion of ds DNA into ss templates relief of chromosome supercoiling Primase synthesis of short primer replacement of RNA with DNA synthesis of most of DNA DNA Polymerase I using RNA as template to make DNA covalent linkage of Okazaki fragments Bacterial Gyrase