QUESTION 27If you mixed SDS with the protein below and ran it in SDS-PAGE, how many bands would you expect to appear on the gel after coomassie staining?-s-s-O 1O 2O 4O too many to count

SDS-PAGE or Sodium Dodecylsulphate- Poly Acrylamide Gel Electrophoresis is a molecular technique to…

Answered: QUESTION 27 If you mixed SDS with the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 3 You are provided with plasmid DNA at 200ng/uL, 10X restriction digestion buffer, restriction enzymes (1 unit/ul) and water. You want to digest 1.5 ug of plasmid DNA with 1 unit (U) of enzyme in 1X buffer in a total volume of 80 ul. Complete the table below: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X 1X DNA 200 ng/ul 1.5 με enzymes 1 unit/ul 1 unit Water Total 80 μL
Question 2 The width of the double helix is 3.4 nm. O True O False
QUESTION 21 Very carefully analyze each nucleotide structures (base and sugars) and identify the structure(s) that is (are) expected to be the part of DNA only. NH NH OH B A O only A O A and C O c and D O only C O A and B
QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O Xgal
QUESTION 36 SDS-PAGE gels are useful in determining the molecular weights of proteins; however, the molecular weights of (one word = adjective) proteins are usually not reliable. %3D
Question 19 Based on the hydropathic index plot below, where in the 3D structure of the protein would you expect to find amino acid number 185? 40 Hydrophobic 20 -20 -40 Hydrophilic 40 60 80 100 120 140 160 180 200 220 240 Residue number Alpha helix Beta sheet O Double helix O Quaternary contact O Interior of the protein O Surface of the protein Hydropathic index 20
Question 15 Three of these are related to a nucleotide. Select the exceptions. R group pentose O phospahte group O nucleoside n amino group
QUESTION 1 The full name of this amino acid is The 3-letter abbreviation is Please also note the following: • The non-ionized structure is shown • Spelling matters • For the acidic amino acids use the full name, e.g. "so-and-so acid" • Case does not matter • Do not include L- or D- in the name НООС- СH - NH2 CH3
QUESTION 11 In this gel image, click on the band that represents the molecule that is smallest in size (mass). Coloctod Coordioator
Question 1.)Draw the main chain structure of a parallel beta sheet that has 4 amino acids in one strand and 5 in the other. Show all of the backbone atoms.Write R1, R2, R3, etc, instead of drawing side chains with the following exception: draw one proline in its entiretywith its side chain. You can put it anywhere in your sheet – I will find it. Add in all hydrogen bonds that stabilize this sheet using dashed lines.
Question 13 of 13 DNA A= 5' GGG GCT AGC CCC 3' DNA B= 3' ATA TAT ATA CCC 5' DNA C= 5' TAC GTT ACG TCG 3' DNA D= 3' ATC TTT GCA TTA 5' The DNA strand whose complementary RNA has the start codon for translation at the 3' end
Question 75 You have isolated a small part of the DNA of a paramecium. The following diagram shows part of the template strand base sequence of the DNA molecule. T-T-T- A - C-G-C-A-T-A–T The coding DNA strand bonded to the DNA template strand illustrated above would have this sequence: O AAATGCGTATA O AAATGCGTATA O AAATATGCGTA O AAACGTATGCGC

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QUESTION 27 If you mixed SDS with the protein below and ran it in SDS-PAGE, how many bands would you expect to appear on the gel after coomassie staining? -s-s- O 1 O2 O 4 O too many to count