radioactively labeled cDNA is used to probe RNA isolated from kidney by Northern blotting. Two bands are seen on the blot. Which of the following reasons is least possible? A. A single pre mRNA is spliced into two different messages B. There are two promoters for the gene C. There are two related genes expressed in kidney D. The mRNA is cleaved in the cytoplasm as part of its degradation Explain why the answer is B or E E. There is a single mRNA produced but some is not polyadenylated

radioactively labeled cDNA is used to probe RNA isolated from kidney by Northern blotting. Two bands are seen on the blot. Which of the following reasons is least possible? A. A single pre mRNA is spliced into two different messages B. There are two promoters for the gene C. There are two related genes expressed in kidney D. The mRNA is cleaved in the cytoplasm as part of its degradation Explain why the answer is B or E E. There is a single mRNA produced but some is not polyadenylated

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Description: radioactively labeled cDNA is used to probe RNA isolated from kidney by Northern blotting. Twobands are seen on the blot. Which of the following reasons is least possible?A. A single pre mRNA is spliced into two different messagesB. There are two pro

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 10QP: The pre-mRNA transcript and protein made by several mutant genes were examined. The results are...

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The mRNA used for the SARS-CoV-2 vaccine from Pfizer/BioNTech is generated in a cell-free system by transcribing a DNA template. The T7 promoter is used as a signal to start transcription. What is the T7 promoter, and give one reason why it is used.
A newly identified protein from the cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding. A possible way this inhibition may be relieved by an sRNA would be: Group of answer choices 1.The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place. 2.The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. 3.The sRNA acts as an enhancer, suppressing the inhibitory protein and allowing translation to take place. 4.The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. Which one is the correct?
The MRNA for a mammalian peptide that enhances electrolyte transfer to reduce paralysis was spliced then reversed transcribed and the CDNA ligated into a phage and transfected into host bacteria cells. The host then transcribed and translated this. The protein product however was non-functional. What is the most probable reason for this result? A. Absence of post-transcriptional modification in bacterial cells B. Differences in the post-translational mechanisms in eukaryotes and prokaryotes. C. Differences in the translational mechanisms involved in prokaryotes, e.g. 70 S ribosome vs 80S ribosome. D. The mRNA did not contain a promoter region to allow peptide translation. E. Insertion of portions of the phage DNA which added sequences for amino acids changing the function of the protein product.
The E. coli lacZYA región will be transcribed at a rate greater than the low basal rate if: A. glucose and lactose are both present in the growth medium, but there's a defect in the cells ability to synthesize cAMP B. there is a defect in binding of the inducer to the product of the lacl gene C. glucose and lactose are both readily available in the growth medium D. the operator has mutated so it can no longer bind repressor E. none of the above
a. What is grey donut shaped object supposed to represent? b. Label with the word "promoter" where one might find the promoter region in this image. c. In the magnified insert, label the template strand that is being transcribed with the word "template". d. In the magnified insert label the 5' and 3' ends of the transcript. e. What entity functions to break the complementary base pairing of a gene at its transcription start site? CS Scanned with CamScanner
The following diagram represents a transcription unit on a DNA molecule. a. Assume that this DNA molecule is from a bacterial cell. Draw the approximate locations of the promoter and terminator for this transcription unit. b. Assume that this DNA molecule is from a eukaryotic cell. Draw the approximate location of an RNA polymerase II promoter.
Explain how the lac operon can be used to express a recombinant protein in an E. coli cell. Explain why the B-galactosidase gene is made in two pieces with the a and 2 parts of the enzyme.
Here is the model for TfR mRNA regulation (see Fig.), for your convenience. Orange protein (IRP or IREBP), when bound to RNA, protects mRNA from degradation. When Fe is bound to the protein, it cannot bind the mRNA. If you made a radioactive DNA probe specific to TfR mRNA and performed a Northern blot analysis (to detect mRNA) from cells treated with high iron (Fe) concentration what would you expect to see? (a) Low iron RNase A (b) High iron O High levels of RNase O Low levels of TfR mRNA Fe RNase O The result would be the same as untreated cells Degradation O High levels of IRP (or IREBP - iron responsive element binding protein) O High levels of TfR mRNA
RNA silencing can be accomplished by specific mRNA degradation or by preventing its translation of the encoded protein. What specific determines which of these two pathways will be followed? It depends on whether the RITS or the RISC complex associates with double-stranded RNA. It depends on whether the single-stranded RNA, associated with RISC, base-pairs perfectly or imperfectly with the target mRNA. It depends on whether Drosha is involved in the process. It depends on whether Dicer is associated with single- or double-stranded RNA. It depends on whether RISC initially binds to single- or double-stranded RNA.
A haploid E. coli cell has the genotype I ¯ P+ O+ Z+ Y ¯ where the I ¯ indicates that the repressor protein cannot bind to the operator due to a mutation. Which of the following statements about cells with this lac operon genotype are correct? I ¯ P+ O+ Z+ Y ¯ Select all that apply. β-galactosidase will be transcribed constitutively. The cells are able to import lactose across the cell membrane. The cells are lac+ and are able to grow on a medium with lactose but no glucose. RNA polymerase can bind to the promoter.
The dlagram below represents the tryptophan operon with the trp leader MRNA transcript enlarged to represent the AUG transiation start codon, two consecutive tryptophan amino acld codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the MRNA leader region. What would happen in this MRNA leader region when cells encounter very low levels of tryptophan in Its environment? Leader region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence mRNA UGGUGG (tryptophan codons) AUG UUUUUU 1 3 4. The translating ribosome would stall at the two tryptophan codons, causing the formation of a hairpin-loop botween regions 3 and 4 to promote transcription of the trp operon. The translating ribosome would stall at the two tryptophan codons causing formation of hairpin-loop between regions 2 and 3, which functions as an anti-lerminator of transcription The translating ribosome would stall at the two tryptophan codons causing formation…
The trp operon is: **(for additional information review the CogBooks Module, answer explained on CogBooks): In the absence of tryptophan, the trp repressor dissociates from the operator, and RNA synthesis proceeds. promoter operator trpE| trpD trpC trpB trpA RNA polymerase repressor When tryptophan is present, the trp repressor binds the operator, and RNA synthesis is blocked. promoter operator trpE trpD trpC trpB trpA RNA polymerase; repressor tryptophan Both negative inducible and positive repressible Positive inducible Negative Repressible O Negative inducible