Researchers can introduce loss-of-function mutations into genesusing the CRISPR/Cas9 technology . Ifyou used this technology to produce the following homozygous loss-of-function mutations in a winter-annual strain of Arabidopsis, describe how each mutation would affect flowering and the requirement for vernalization.A. FLCB. VIN3C. COLDAIR

Researchers can introduce loss-of-function mutations into genesusing the CRISPR/Cas9 technology . Ifyou used this technology to produce the following homozygous loss-of-function mutations in a winter-annual strain of Arabidopsis, describe how each mutation would affect flowering and the requirement for vernalization.A. FLCB. VIN3C. COLDAIR

Name: Researchers can introduce loss-of-function mutations into genesusing t
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: Researchers can introduce loss-of-function mutations into genesusing the CRISPR/Cas9 technology . Ifyou used this technology to produce the following homozygous loss-of-function mutations in a winter-annual strain of Arabidopsis, describe how each mu

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 6RQ: What is the role of Agrobacterium tumefaciens in the production of transgenic plants? a. Genes from...

See similar textbooks

Similar questions

To produce transgenic plants, plant tissue is exposed to Agrobacteriumtumefaciens and then grown in media containing kanamycin, carbenicillin, and plant growth hormones. Explain the purpose behind each of these three agents. What would happen if you left out the kanamycin?
Which of the following BEST states genome editing in plants? A. transform large DNA constructs by suppression and activation B. insert a functional cis-regulatory element in the natural genes transgene vector C. assemble and synthesize large DNA molecules in a single transgene vector D. deletion, mutation, or integration of the gene of interest depending on the target trait
Bacterial glucuronidase converts a colorless substance called X-Gluc into a bright blue indigo pigment. The gene for glucuronidase also works in plants if given a plant promoter region. How would you use this gene as a reporter gene to find the tissues in which a plant gene that you have just cloned is normally active? (Assume that X-Gluc is easily taken up by the plant tissues.)
Explain the difference between gene modification and gene addition.Are the following examples of gene modification or gene addition?A. A mouse model to study cystic fibrosisB. Introduction of a pesticide-resistance gene into corn using theT-DNA vector of A. tumefaciens
You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. You conduct the screen and find two different plants that show the same mutant phenotype. You then use a complementation test. What is the predicted outcome of this test if both phenotypes are caused by mutations in separate genes? recover the wild type phenotype overexpress the gene O recover the mutant phenotype
Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools. Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (TO-34) containing one copy of crylla detected by Southern blot. Protein was expressed in high concentration at 45 davs after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A…
Trace the genetic steps in the development of a peanut plant that isresistant to insect larvae (table given). (Hint: You need to useAgrobacterium.)
In roses, the synthesis of red pigment is produced by two steps in a pathway. gene O magenta intermediate - gene P colorless intermediate- red pigment What would the phenotype be of a plant homozygous for a null mutation of gene P? What would the phenotype be of a plant homozygous for a null mutation of gene Q? What would the phenotype be of a plant homozygous for null mutations of genes P and Q? magenta red Match a genotype to each strain. colorless Strain P locus Q locus homozygous null mutation of gene P homozygous null mutation of gene Q homozygous null mutations of genes P and Q Answer Bank plp PIP What F2 ratio is expected from crossing a plant that is homozygous for a null mutation of gene P with a plant that is homozygous for a null mutation of gene Q? Assume independent assortment. 9 colorless : 4 magenta : 3 red 9 red : 4 colorless : 3 magenta O 9 red : 4 magenta : 3 colorless
How reliable does the rbcL gene work as a barcode for identifying plant species?
a) How are mutations beneficial for plant breeding? Discuss with an example b) Explain in brief the technology that made us self-sufficient in food production.
You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. Given what you know about photoperiodism in Arabidopsis, what phenotype are you looking for and under what photoperiodic conditions would you perform the experiment? delayed flowering in long days delayed flowering in short days same flowering in short days early flowering in short days same flowering in long days early flowering in long days
After much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2. The gene associated with the mut1 mutation is called COB1 (i.e. mutations in COB1 cause the mut1 phenotype.) The researcher extracts DNA and RNA from developing ears of wild type, mut1 and mut2 plants. From the DNA samples COB1 PCR primers are used to amplify the coding region of the COB1 gene (see DNA gel below). An RNA gel is used to examine the expression of COB1 all three plants (see RNA gel below). results in photo: Which of the following is TRUE about the COB1 gene. a. There is a deletion in the COB1 gene in the mut1 plants. b. COB1 is not transcribed in the mut2 plant. c. There is a deletion in the COB1 gene in the mut2 plants. d. COB1 is not transcribed in the mut1 plant.