Serum is diluted with an equal amount of diluent (such as tube #1, 1/2 and tube #2, 1/2). What is the concentration in tube #2 if the original concentration was 100 mg/dL?
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Serum is diluted with an equal amount of diluent (such as tube #1, 1/2 and tube #2, 1/2). What is the concentration in tube #2 if the original concentration was 100 mg/dL?
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- If you make a five-tube twofold dilution using 2 mL of serum, what is the concentration of serum in tube #4?Tube A is a 1/5 dilution and then, using Tube A, is diluted to make a 1/10 labeled Tube B. What is the dilution to be made for each tube, is using serum and saline? What is the dilution of the solution in tube 1 and 2 .Yasuo Yamagata, a 63-year-old, experienced severe back pain for several weeks before visiting his family physician. He also complained of fatigue and looked pale. Blood analysis revealed a red blood cell count of 3.2 × 106/μl (normal 4.2–5.0 × 106/μl), a white blood cell count of 2800/μl (normal 5000/μl), a sedimentation rate of 30 mm/h (normal <20 mm/h) and a serum IgG of 4500 mg/dl (normal 600–1500 mg/dl). IgA and IgM levels were well below normal. Skeletal survey showed lytic lesions in vertebrae, ribs and skull. A bone marrow sample revealed 75% infiltration with plasma cells. Elevated protein in urine was confirmed to be Bence-Jones protein (immunoglobulin light chains). The patient was diagnosed with IgG λ multiple myeloma and began an immediate chemotherapy regime. Which of the following would be consistent with this type of malignant tumor of plasma cells? a. Serum IgG is polyclonal. b. Anemia and neutropenia are present as the result of plasma-cell infiltration in the…
- Give the instructions for preparing a two-fold serial dilution of serum from 0.5 mL serum and using 0.5mL saline in each of five numbered tubesWhat is Thalassemia and Hemolytic disorder? Differentiate. Name TWO (2) methods that are applicable for serum preservation? Explain TWO importance of studying Blood transfusion? Give FOUR (4) therapeutic uses of serum? conclusionWhy is the Erythrocyte Sedimentation Rate ineffective in patients with severe anemia? Identify an appropriate sedimentation test that could be performed alternatively.
- Draw a standard curve (using linear graph paper) of A595 (Y axis) versus μg protein for tubes 1-6 (standards) and use this to determine the amount of protein in each of the fractions (serum, B and crude IgG). Calculate the total amount of protein in the starting serum and the amount recovered in each of fraction B and the IgG fraction (you need to know the total volumes of these samples). The volume recorded for fraction c was 1ml and fraction b was 11ml. Please I need helpGiven that the normal range for serum protein is about 7 g/100ml and albumin is about 4 g/100ml, what clinical conclusions can you draw from the following samples A-C? If samples are not normal, what protein fraction is increased/decreased and with what disease is this protein pattern likely to be associated? Sample A: total protein = 14 g/100mlalbumin= 8 g/100 ml Sample B:total protein = 5.8 g/100ml albumin = 4 g/100 ml Sample C:total protein = 3.5 g/100mlalbumin = 2g/100 mlA serum is diluted 1:20 then 2 parts of the resulting dilution is added to 40 parts saline. Then 3 parts of the last dilution was added to saline to have a total volume of 6 parts. What is the final dilution?