Step 7: Determine the appropriate calculation for the pl of lysine. For lysine, the pK, of the amine is 8.95, while the pK of the carboxylic acid is 2.18 and the pK of the amine in the side chain is 10.53. Calculate the pl of lysine.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Question 5 of 40
<>
Side-by-Side
Identify the charge states for aspartic acid (Asp), leucine
(Leu) and Lysine (Lys) at pH 1, 7 and 12. Then calculate
the isoelectric point (pI).
Step 7: Determine the appropriate calculation for the pl
of lysine.
For lysine, the pKa of the amine is 8.95, while the pKa of
the carboxylic acid is 2.18 and the pK, of the amine in the
Strategy: First identify the sidechain on each amino acid.
Then determine whether the side chain is acidic, basic or
side chain is 10.53.
neutral. Determine whether each functional group is
Calculate the pI of lysine.
charged or not at the pH indicated. Calculate the (pI) from
the pKa values of the functional groups.
pI
MacBook Pro
Transcribed Image Text:Question 5 of 40 <> Side-by-Side Identify the charge states for aspartic acid (Asp), leucine (Leu) and Lysine (Lys) at pH 1, 7 and 12. Then calculate the isoelectric point (pI). Step 7: Determine the appropriate calculation for the pl of lysine. For lysine, the pKa of the amine is 8.95, while the pKa of the carboxylic acid is 2.18 and the pK, of the amine in the Strategy: First identify the sidechain on each amino acid. Then determine whether the side chain is acidic, basic or side chain is 10.53. neutral. Determine whether each functional group is Calculate the pI of lysine. charged or not at the pH indicated. Calculate the (pI) from the pKa values of the functional groups. pI MacBook Pro
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