Substance A and B were found to have retention times of 16.40 and 17.63 min. respectively, on a 30cm HPLC column. An unretained species passed through the column in 1.30 min. The peak widths at base for A and B were 1.11 and 1.21 min, respectively. Calculate the a.) Column resolution b.) the average nbumber of plates in the column and the plate height.
Substance A and B were found to have retention times of 16.40 and 17.63 min. respectively, on a 30cm HPLC column. An unretained species passed through the column in 1.30 min. The peak widths at base for A and B were 1.11 and 1.21 min, respectively. Calculate the a.) Column resolution b.) the average nbumber of plates in the column and the plate height.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.16QAP
Related questions
Question
Substance A and B were found to have retention times of 16.40 and 17.63 min. respectively, on a 30cm HPLC column. An unretained species passed through the column in 1.30 min. The peak widths at base for A and B were 1.11 and 1.21 min, respectively.
Calculate the
a.) Column resolution
b.) the average nbumber of plates in the column and the plate height.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 4 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning