Table 1: Absorbance of Ibuprofen at 221 nm in phosphate buffer (pH 6.8) Concentration of Absorbance ibuprofen (ug/mL) 1 0.063 2 0.104 3. 0.134 0.178 0.222 6. 0.269 7 0.308 8 0.342 9. 0.386 10 0.433 a) Plot the values in table 1 on the graph paper provided and draw a line of best fit. Determine the equation of the straight line (y = mx + c) b) {x} mg of ibuprofen bulk powder was transferred to a 100 mL volumetric flask and made up to volume with buffer. 1 mL of the solution was further diluted to 100 mL. Use the data in a) to calculat the percentage purity of bulk ibuprofen powder, if the absorbance of the diluted solution is o.219.
Table 1: Absorbance of Ibuprofen at 221 nm in phosphate buffer (pH 6.8) Concentration of Absorbance ibuprofen (ug/mL) 1 0.063 2 0.104 3. 0.134 0.178 0.222 6. 0.269 7 0.308 8 0.342 9. 0.386 10 0.433 a) Plot the values in table 1 on the graph paper provided and draw a line of best fit. Determine the equation of the straight line (y = mx + c) b) {x} mg of ibuprofen bulk powder was transferred to a 100 mL volumetric flask and made up to volume with buffer. 1 mL of the solution was further diluted to 100 mL. Use the data in a) to calculat the percentage purity of bulk ibuprofen powder, if the absorbance of the diluted solution is o.219.
Chapter31: Introduction To Analytical Separations
Section: Chapter Questions
Problem 31.17QAP
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Transcribed Image Text:Preparation of Ibuprofen calibration curve
Accurately weighed ibuprofen standard (100 mg) was transferred to a 100 ml volumetric flask and
dissolved in phosphate buffer solution (pH 6.8). The final volume was adjusted to 100 ml with buffer
solution (soln A). One millilitre of soln A was transferred to another volumetric flask and diluted with
buffer solution up to 100 ml to get soln B. Solution B was further diluted to obtained calibration
solutions in the range o to 10 ug/mL. The absorbance of each of the calibration standards were
recorded at 221 nm to give the data in table 1.
Table 1: Absorbance of Ibuprofen at 221 nm in phosphate buffer (pH 6.8)
Concentration of
Absorbance
ibuprofen (ug/mL)
0.063
0.104
0.134
0.178
1
2.
4.
0.222
9.
0.269
7.
0.308
8.
0.342
6.
0.386
10
0.433
a) Plot the values in table 1 on the graph paper provided and draw a line of best fit. Determine the
equation of the straight line (y = mx + c)
b) {x} mg of ibuprofen bulk powder was transferred to a 100 mL volumetric flask and made up to
volume with buffer. 1mL of the solution was further diluted to 100 mL. Use the data in a) to calculate
the
percentage purity of bulk ibuprofen powder, if the absorbance of the diluted solution is 0.219.
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