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Table 3- Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Tube # 2c 3c 4c 5c Potato Expt. Temp. extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C Boiling 100 °C dH₂O Catechol (mL) (mL) 。T 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 Subtract 2nd from 3rd absorbance 0.031 -0.018 10.016 -0.011

Table 3- Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Tube # 2c 3c 4c 5c Potato Expt. Temp. extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C Boiling 100 °C dH₂O Catechol (mL) (mL) 。T 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 Subtract 2nd from 3rd absorbance 0.031 -0.018 10.016 -0.011

3-2-1 Code It
3-2-1 Code It
6th Edition
ISBN:9781337660549
Author:GREEN
Publisher:GREEN
Chapter17: Cpt Pathology And Laboratory
Section: Chapter Questions
Problem 15MC
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Table 3-Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: Absorbance: 25 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 Subtract 2nd from 3rd absorbance 0.031 1-0.018 10.016 -0.011
Transcribed Image Text:Table 3-Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: Absorbance: 25 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 Subtract 2nd from 3rd absorbance 0.031 1-0.018 10.016 -0.011
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