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- The best definition for resolution is: O The ability of an organelle to pick up stain The thickness of a specimen to be examined by microscopy The observed size divided by the actual size O The magnification of a microscope The distance between two distinguishable objectsWhich of the following is an advantage of confocal microscopy over conventional fluorescence microscopy? The interaction of the laser beam with the cell surface allows the imaging of individual macromolecules O Only out-of-focus light enters the microscope lens, making the object appear illuminated against a dark background The use of electrons instead of light to image the specimen results in greatly increased resolving power O Optical sections in a single plane of focus can be visualized at different depths in the specimen The natural contrast of an unstained specimen is enhancedWhich of the following is never useful for observing living cells? O phase-contrast microscope scanning electron microscope O darkfield microscope O brightficld microscope confocal microscope
- Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organellesStaining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4X
- In order to visualize the fine structure of viruses and cytoskeletal filaments at 10-25 nanometers in diameter the type of microscopy that would be most effective is O standard light microscopy O phase-contrast light microscopy transmission electron microscopy O darkfield light microscopy O differential-interference microscopyFor each type of microscopy, know how each is used and the type of image produced. kEEP IT SHORT AND SWEET Light microscope – Transmission electron microscope - Scanning electron microscope –A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 103 mm) in diameter, and wants to view a magnified image of a %3D cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? O 10X O 40X O 100X O 4X Question 7 2 pts
- What description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.There are many different types of microscopes that can be used in science. Research the function of each type of microscope below Stereomicroscopes Bright field microscope Dark field microscope Fluorescence microscope Phase contrast microscope Nomarski microscope Scanning electron microscope Transmission electron microscopeList and define the three properties of properly working microscope.
