The amino acid histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, as shown. COOH H₂N-CH CH. H pH = H CH lonizable -COOH group COO H₂N-CH 1.8 pk, H COO CH -HisH+ COO™ H₂N-CH 6.0 pk₂ CH₂ H H -His CH -NH+ COO™ H₂N-CH 9.2 pk3 CH₂ H -N H N -NH₂ CH A biochemist makes up 80 mL of a 0.11 M solution of histidine at a pH of 5.1. She then adds 60 mL of 0.10 M HCl. What is the pH of the resulting solution?

Introduction to General, Organic and Biochemistry
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ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter23: Enzymes
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Problem 23.65P
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The amino acid histidine has ionizable groups with pKą values of 1.8, 6.0, and 9.2, as shown.
COOH
T
H₂N-CH
|
CH₂ H
-O
pH
UT
=
ZI
Ionizable -COOH
group
CH
H₂N-CH
T
1.8
COO-
pk₁
CH2
UI
COO™
H
IZ
CH
-HisH+
COO™
+
H₂N-CH
6.0
pK₂
CH ₂
UI
-His
H
CH
-NH
COO™
H₂N-CH
9.2
pk3
CH ₂
UI
—NH₂
H
A biochemist makes up 80 mL of a 0.11 M solution of histidine at a pH of 5.1. She then adds 60 mL of 0.10 M HCl. What is
the pH of the resulting solution?
CH
Transcribed Image Text:The amino acid histidine has ionizable groups with pKą values of 1.8, 6.0, and 9.2, as shown. COOH T H₂N-CH | CH₂ H -O pH UT = ZI Ionizable -COOH group CH H₂N-CH T 1.8 COO- pk₁ CH2 UI COO™ H IZ CH -HisH+ COO™ + H₂N-CH 6.0 pK₂ CH ₂ UI -His H CH -NH COO™ H₂N-CH 9.2 pk3 CH ₂ UI —NH₂ H A biochemist makes up 80 mL of a 0.11 M solution of histidine at a pH of 5.1. She then adds 60 mL of 0.10 M HCl. What is the pH of the resulting solution? CH
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