The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m 6- RNA Absorbance at 260 (µg) 0.00 10.29 20.58 30.87 41.16 mass= nm 0.227 0.452 0.786 1.078 1.296 1g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. ug

The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m 6- RNA Absorbance at 260 (µg) 0.00 10.29 20.58 30.87 41.16 mass= nm 0.227 0.452 0.786 1.078 1.296 1g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. ug

Living by Chemistry

2nd Edition

ISBN:9781464142314

Author:Angelica M. Stacy

Publisher:Angelica M. Stacy

ChapterU5: Fire: Energy , Thermodynamics, And Oxidation-reduction

SectionU5.116: How Absorbing: Spectroscopy

Problem 6E

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The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b = RNA (µg) 0.00 10.67 21.34 32.01 42.68 mass= Absorbance at 260 nm 0.224 0.512 0.769 1.145 1.465 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.636. µg µg
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