The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa. Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture). 1: Standard Protein Ladder 2: Protein X solution 3: Protein X solution + β-mercaptoethanol Based on the given gel profile in the picture, can it be concluded that the solution of Protein X is pure? Explain. can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.
The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa. Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture). 1: Standard Protein Ladder 2: Protein X solution 3: Protein X solution + β-mercaptoethanol Based on the given gel profile in the picture, can it be concluded that the solution of Protein X is pure? Explain. can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.
Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter34: Tomography And Digital Tomosynthesis
Section: Chapter Questions
Problem 4RQ
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The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa. Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture).
1: Standard Protein Ladder
2: Protein X solution
3: Protein X solution + β-mercaptoethanol
Based on the given gel profile in the picture,
- can it be concluded that the solution of Protein X is pure? Explain.
- can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.
![200 kDa
150 kDa
100 kDa
75 kDa
50 kDa
25 kDa
1
2
3
|||](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F530198a4-a3a9-4348-8922-ab4aaa2d4212%2F15671eaf-1a67-4329-a476-907526f19c41%2Fackm72c_processed.png&w=3840&q=75)
Transcribed Image Text:200 kDa
150 kDa
100 kDa
75 kDa
50 kDa
25 kDa
1
2
3
|||
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