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Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml Chloroform-treated lysate Dilution 10-³ [[ ]]] 9ml 104 10-4 10-5 9ml 10-1 9ml 10-² Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 9ml 103 ↓↓ 0.1 ml DO 0.1 ml 9ml 10.5 0.1 ml +0.5 ml E. coli AA Incubate O/N at 35°C, observe for plaques Molten soft agar overlay ↓ OO B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23

Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml Chloroform-treated lysate Dilution 10-³ [[ ]]] 9ml 104 10-4 10-5 9ml 10-1 9ml 10-² Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 9ml 103 ↓↓ 0.1 ml DO 0.1 ml 9ml 10.5 0.1 ml +0.5 ml E. coli AA Incubate O/N at 35°C, observe for plaques Molten soft agar overlay ↓ OO B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23
Transcribed Image Text:Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23
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