There are two preparations of purified pyruvate dehydrogenase complex enzymes with all therequired cofactors. You add pyruvate to each preparation and then measure the rate of productionof acetyl-CoA and acetaldehyde under aerobic conditions.Acetyl-CoA(molecules/sec)10Free acetaldehyde(molecules/sec)106Preparation APreparation B1010How might the pyruvate dehydrogenase complex enzymes differ in each preparation?

Pyruvate DeHydrogenase (PDH) enzyme complex is a large molecule (of size bigger than a ribosome)…

Answered: There are two preparations of purified…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter20: Electron Transport And Oxidative Phosphorylation

Section: Chapter Questions

Problem 7P

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Complete oxidation of a 16-carbon fatty acid can yield 129 molecules of ATP Study Figure 19.2 and determine how many ATP molecules would be generated if a 16-carbon fatly acid were metabolized solely by the TCA cycle, in the form of S acetyl-CoA molecules.
GTP or ATP is produced during the conversion of isocitrate into ketoglutarate succinyl CoA into succinate fumarate into malate malate into oxaloacetate
Comparing Glycolysis Entry Points for Sucrose Sucrose can enter glycolysis by either of two routes: Sucrose phosphorylase: Sucrose + Pi fructose + glucose-1-phosphate Invertase: Sucrose + H20 fructose + glucose Would either of these reactions offer Jin advantage over the other In the preparation of hexoses fur entry into glycolysis?
The Effect of lodoacetic Acid on the Glyceraldehyde-3-P Dehydrogenase Reaction (Integrates with Chapters 4 and 14.) How might iodoacetic acid affect the glyceraldehydes-3-phosphate dehydrogenase reaction in glycolysis? Justify your answer.
Radiotracer Labeling of Pyruvate from Glucose Determine the anticipated location in pyruvate of labeled carbons If glucose molecules labeled (in separate experiments.) with 14C at each position of (.tie carbon skeleton proceed through the glycolytic pathway.
Arrange the steps of breakdown of oleic acid (pictured below) in the correct order. 1 2 3 4 5 activation by acyl-CoAsynthe ✓ [Choose ] one cycle ofß-oxidationbeginning at the enoyl-CoAhydratasestep three cycles ofẞ-oxidation five cycles ofß-oxidation activation by acyl-CoAsynthetase enoyl-CoAisomeraseactivity enoyl-CoAisomeraseactivity V one cycle ofß-oxidationbegini V five cycles ofß-oxidation OH
Complete the balanced equation for the synthesis of the fatty acid. x Acetyl-CoA + y ATP + z NADPH + z H+ CH3(CH2)20COOH + x CoA+ y ADP + y Pi + v H₂O + Z NADP+ x=12 y=11 z=22 v=10 x=5 y=4 Z=8 v=3 x=7 y=6 Z=12 v=5 x=11 y=10 z=20 v=9
B1. Complete the Kreb's Cycle Acetyl CoA Citric Acd Malate dehydrogenase NADH NAD Isocitrate NAD Isocitrate Fumarase dehydrogenase NADH Fumarate NAD" FAD COASH Succinate dehydrogenase Succinate Succinyl CoA Succinyl CoA synthetase B2. How many ATP molecules can be produced from 4 molecules of Acetyl CoA if all of them enter the Kreb's Cycle? Assume all the energy yielding products enters the electron transport chain.
Upon considering the complete oxidation of one mole of fatty acyl (CoA) of an arachidic acid (20:0). Answer the following lettersa. total no. of NADH produced from the oxidation of all acetyl CoA in the acetyl cycleb. otal no. of FADH2 produced from the oxidation of all acetyl CoA in the acetyl cyclec. total atp yield
int IMLeagues | Provi... Time Remaining: 00:18:21 A Hide Time Remaining A Arsenic is a water soluble element that is a poison because it functions as an allosteric inhibitor of the citric acid cycle enzymes pyruvate dehydrogenase and succinate dehydrogenase. One of the consequences of arsenic poisoning is lactic acidosis from the buildup of lactate in the body. Why does arsenic treatment result in a buildup of lactate? Legend Pyruvate Acetyl Hydrogen COA SH + NAD+ Pyruvate dehydrogenase Co,+NADH, H* Adenosine Carbon ATR triphosphate CoA Охygen Guanosine CGTP triphosphate Sulfur Acetyl-CoA COA SH Q Coenzyme Q CoA Coenzyme A HCO3 +CATP Water NADH Nicotinamide adenine dinucleotide Citrate Pyruvate carboxylase Pyruvate dehydrogenase Enzyme ADP+Pi Citrate synthase Aconitase Oxaloacetate Water cis-Aconitate Water NADH, H* Aconitase Malate dehydrogenase NAD. D-Isocitrate NAD* Malate NADH, H* Isocitrate dehydrogenase CO2 Citric acid cycle Fumarase MacBook Pr
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Which enzyme is used for both the synthesis and breakdown of acetoacetate? thiolase O HMG-CoA synthase OB-hydroxybutyrate dehydrogenase O B-Ketoacyl-CoA transferase

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