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To make 200 ml 0.4XSSC (Saline and Sodium Citrate) solution for FISH post-washing, how many ml 20X SSC solution is needed?
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- To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?
- How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? PLEASE GIVE THE MEASUREMENTSHow many mL of 10 x stock solution would you need to make 118 mL of 1x PBS? Report your answer to 1 decimal place.You need to make 20 mL of a 1/100 (10-2) dilution of a crystal violet solution? How much diluting fluid will be required? Note: Your answer should be in numerical format to the nearest 0.1 mL.
- You receive an order for 24 mEq calcium gluconate in 50 mL normal saline. What volume, in mL, of calcium gluconate 10% stock solution is needed to provide the number of mEq ordered? Cation Aluminum Ammonium Calcium Ferric Ferrous Lithium Magnesium Potassium Sodium Formula Valence Formula weight A13+ 3 1 2 3 2 1 2 1 1 NH₂+ Ca²+ Fe³+ Fe2+ Li+ Mg2+ K+ Na+ 27 18 40 56 56 7 24 39 23 Anion Acetate Bicarbonate Carbonate Chloride Citrate Gluconate Hydroxide Lactate Phosphate, monobasic Phosphate dibasic Sulfate Formula Valence C₂H30₂ 1 HCO3 1 CO3²- 2 CI- 1 3 1 1 1 1 C6H5O7³- C6H₁107 OH- C6H5O3 H₂PO4 HPO4²- SO₂²- 2 2 Formula weight 59 61 60 35.5 189 195 17 89 97 96 96The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).
- 50 mL of 5mM NaCl solution from 1 M NaCl solution3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLIf a pharmacist needs to prepare a bacterial suspension with a concentration of 2.4x10^2 cfu using 1 mL of 2.4 x 10^4 bacterial suspension, what size of volumetric flask will she/he be needing?