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Q: The thickness of a specimen in view is known as the _______________.
A: Depth of focus.
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True or False:
Ultrathin sections are used for light microscopy but are to thin to be stable under an electron mocroscope X-ray beam.
Microscopes are used to visualize objects that are not visible to the naked eye. In microbiology, microscopes are used to visualize cells, small molecules, and microorganisms. There are different types of microscopes like a light microscope and an electrical microscope. The microscope uses the optical system for magnification
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- The diffraction limit is an unbeatable barrier limiting the effective resolution of light microscopy due to the inherent wavelength of light.True or FalseTrue or false : each objective of a phase contrast microscope contains a phase plate. phase contrast microscopy relies on the condenser to increase the contrast of an image. in brightfield microscopy, closing the condenser aperture will decrease the contrast of the speciment. stained cell speciments are best viewed by phase contrast microscopy.Comparison of the different objectives of the compound microscope Point of Comparison Scanner LPO НРО O1O Degree of magnification (X) 4х 10x 40x 100x Details of the specimen (least to most detailed) Area of field of view (smallest to biggest) Brightness of image (dimmest to brightest ) Working distance (mm)
- Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMExplain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?
- In microscopy, why are different stains used for different samples? Be specific.Between wet mount and hanging drop slide preparations, which is more resistant to evaporation? Which works best with phase-contrast microscopy?Artefacts seen in virtual slides of given data below. Microscopy not radiology.
- True or False: The microscope should be stored with the oil immersion lens in position over the stage. If this statement is false, correct the statement.Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.