Two substances A and B have retention times of 16.40 and 17.63 min respectively on 30.0 cm column. An unsuccessful species passed over the column in 1.30 min. The widths (at the base) of the peaks ofA and B are respectively 1.11 min and 1.21 min: Calculate: a) The Resolution Rs of the column. b) The average number (Nav of theoretical plates in the column c) The equivalent height (H) d) length of column required to achieve a resolution of 1.5, e) time required to elute substance B on the longer column.

Two substances A and B have retention times of 16.40 and 17.63 min respectively on 30.0 cm column. An unsuccessful species passed over the column in 1.30 min. The widths (at the base) of the peaks ofA and B are respectively 1.11 min and 1.21 min: Calculate: a) The Resolution Rs of the column. b) The average number (Nav of theoretical plates in the column c) The equivalent height (H) d) length of column required to achieve a resolution of 1.5, e) time required to elute substance B on the longer column.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.17QAP: From the data in Problem 26-14, calculate for species C and D) (a) the resolution. (b) the length of...

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